Spring Viremia of Carp Virus Infection Induces Carp IL-10 Expression, Both In Vitro and In Vivo
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses, Cells and Fish
2.2. Reagents
2.3. Stimulation of EPC and cHK Cells with SVCV Infection
2.4. SVCV Inoculation of Carp
2.5. Inhibition of Signal Transduction Pathways
2.6. Quantification of Carp IL-10 Gene Expression Using RT-qPCR
2.7. Quantification of IL-10 Protein Expression Using ELISA
2.8. Statistical Analysis
3. Results
3.1. SVCV Infection Induced Carp IL-10 Production at mRNA and Protein Levels in EPC and cHK Cells
3.2. SVCV Infection Induced Carp IL-10 Production at mRNA and Protein Levels in Carp Tissues
3.3. Inhibition of JAK-STAT, NF-κB and p38MAPK Pathways Hindered the Upregulation of Carp IL-10
3.4. JAK1, JAK2, JAK3, TYK2, STAT5 and STAT6 Were Involved in SVCV-Induced Carp IL-10 Expression
4. Discussions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Primers | Sequence (5′–3′) | Accession No. |
---|---|---|
β-actin-F | GACCTGTATGCCAACACTGTAT | M24113 |
β-actin-R | TCCTGCTTGCTAATCCACATC | |
IL-10-F | AGTCCTTATGGCTGTCACGTCATG | AB110780 |
IL-10-R | TTGAGTGCAAGTGGTCCTTCTGG | |
JAK1-F | AGGGGACACCTCTACTGGATGC | AH004872 |
JAK1-R | GTGTGAGAAGTTACGCTGCTTA | |
JAK2-F | CGGAGTGTCACCAGTCTAC | KJ782027 |
JAK2-R | GGTAAGACATAACACAGTCATCC | |
JAK3-F | GGCATCAGAGGACCTTTCATAC | AF148993 |
JAK3-R | CTGCCATTCCCAAGCATTCCTG | |
TYK2-F | TGAGGGTGAGGTGACTGCTGAAG | KJ782031 |
TYK2-R | AGCGGTTCCTTTTCACCTAATCC | |
STAT5-F | CCTGGGATGGAATAGATTTAGAG | KJ782032 |
STAT5-R | GACAGCGGTCATACGTGCTCTTTAG | |
STAT6-F | AATGACCCCGCAGTTACAGTTC | KJ782033 |
STAT6-R | GTTGTAGTTTGACCCTCTCCAC |
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Ouyang, P.; Tao, Y.; Wei, W.; Li, Q.; Liu, S.; Ren, Y.; Huang, X.; Chen, D.; Geng, Y. Spring Viremia of Carp Virus Infection Induces Carp IL-10 Expression, Both In Vitro and In Vivo. Microorganisms 2023, 11, 2812. https://doi.org/10.3390/microorganisms11112812
Ouyang P, Tao Y, Wei W, Li Q, Liu S, Ren Y, Huang X, Chen D, Geng Y. Spring Viremia of Carp Virus Infection Induces Carp IL-10 Expression, Both In Vitro and In Vivo. Microorganisms. 2023; 11(11):2812. https://doi.org/10.3390/microorganisms11112812
Chicago/Turabian StyleOuyang, Ping, Yu Tao, Wenyan Wei, Qiunan Li, Shuya Liu, Yongqiang Ren, Xiaoli Huang, Defang Chen, and Yi Geng. 2023. "Spring Viremia of Carp Virus Infection Induces Carp IL-10 Expression, Both In Vitro and In Vivo" Microorganisms 11, no. 11: 2812. https://doi.org/10.3390/microorganisms11112812