Clinical Evaluation and Comparison of Two Microfluidic Antigenic Assays for Detection of SARS-CoV-2 Virus
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Positive Samples | Negative Samples | Total | ||||
---|---|---|---|---|---|---|
N. (%) | 51 (33.8%) | 100 (66.2%) | 151 (100%) | |||
Ct Values of positive samples | ||||||
Mean | Median | Standard Deviation (SD) | Interquartile Range (IQR) | Min | Max | |
Overall | 26.0 | 27.0 | 5.9 | 10.3 | 17.0 | 35.0 |
Very high | 19.2 | 18.5 | 2.7 | 3.5 | 17.0 | 25.0 |
High | 28.4 | 28.5 | 1.7 | 2.7 | 25.5 | 30.0 |
Moderate | 34.1 | 34.5 | 0.9 | 0.5 | 32.0 | 35.0 |
Accuracy | Specificity | Cohen’s Kappa Coefficient | |
---|---|---|---|
LumiraDx SARS-CoV-2 Ag Ultra vs. RT-qPCR | |||
Overall | 54.90% | 100.00% | 0.62 |
Very high | 94.74% | 0.97 | |
High | 43.48% | 0.56 | |
Moderate | 0.00% | 0.00 | |
LumiraDx SARS-CoV-2 & Flu A/B vs. RT-qPCR | |||
Overall | 45.10% | 100.00% | 0.52 |
Very high | 94.74% | 0.97 | |
High | 21.74% | 0.31 | |
Moderate | 0.00% | 0.00 |
SARS-CoV-2 Ultra (N. Pos) | SARS-CoV-2 & Flu A/B (N. Pos) | Cohen’s Kappa Coefficient | |
---|---|---|---|
Overall | 28 | 23 | 0.88 |
Very high | 18 | 18 | 1.00 |
High | 10 | 5 | 0.64 |
Moderate | 0 | 0 | 0.00 |
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Bottino, P.; Pizzo, V.; Castaldo, S.; Scomparin, E.; Bara, C.; Cerrato, M.; Sisinni, S.; Penpa, S.; Roveta, A.; Gerbino, M.; et al. Clinical Evaluation and Comparison of Two Microfluidic Antigenic Assays for Detection of SARS-CoV-2 Virus. Microorganisms 2023, 11, 2709. https://doi.org/10.3390/microorganisms11112709
Bottino P, Pizzo V, Castaldo S, Scomparin E, Bara C, Cerrato M, Sisinni S, Penpa S, Roveta A, Gerbino M, et al. Clinical Evaluation and Comparison of Two Microfluidic Antigenic Assays for Detection of SARS-CoV-2 Virus. Microorganisms. 2023; 11(11):2709. https://doi.org/10.3390/microorganisms11112709
Chicago/Turabian StyleBottino, Paolo, Valentina Pizzo, Salvatore Castaldo, Elisabetta Scomparin, Cristina Bara, Marcella Cerrato, Sabrina Sisinni, Serena Penpa, Annalisa Roveta, Maria Gerbino, and et al. 2023. "Clinical Evaluation and Comparison of Two Microfluidic Antigenic Assays for Detection of SARS-CoV-2 Virus" Microorganisms 11, no. 11: 2709. https://doi.org/10.3390/microorganisms11112709