Sequencing of SARS-CoV-2 RNA Fragments in Wastewater Detects the Spread of New Variants during Major Events
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Extraction of Total Nucleic Acid from Wastewater Samples
2.3. Sequencing
2.4. Variant Mapping
3. Results
3.1. Spread of Omicron BA.1 during International Sports Competitions in December 2021
3.2. The Increased Relative Prevalence of Omicron BA.1 Caused Higher Infection Rates
3.3. No Effect of the WEF in May 2022 on the Relative Prevalence of the Dominant Omicron Variants
3.4. Arrival and Spread of Omicron BA.2.75 during WEF 2023 in Davos
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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8 November 2021–22 November 2021 | ||
Variant | Num seq | Frequency |
21J (Delta) | 307 | 0.95 |
21I (Delta) | 14 | 0.04 |
21A (Delta) (B.1.617.2) | 1 | 0.00 |
22 November 2021–6 December 2021 | ||
Variant | Num seq | Frequency |
21J (Delta) | 241 | 0.96 |
21I (Delta) | 8 | 0.03 |
21K (Omicron) (BA.1) | 3 | 0.01 |
6 December 2021–20 December 2021 | ||
Variant | Num seq | Frequency |
21J (Delta) | 163 | 0.67 |
21K (Omicron) (BA.1) | 76 | 0.31 |
21I (Delta) | 3 | 0.01 |
20 December 2021–3 January 2022 | ||
Variant | Num seq | Frequency |
21K (Omicron) (BA.1) | 410 | 0.86 |
21J (Delta) | 66 | 0.14 |
21I (Delta) | 1 | 0.00 |
Asia | Eastern Asia | South Korea | 0.53 |
Mauritius | 0.45 | ||
South-eastern Asia | Viet Nam | 0.92 | |
Thailand | 0.84 | ||
Cambodia | 0.44 | ||
Singapore | 0.19 | ||
Malaysia | 0.14 | ||
Western Asia | Georgia | 0.65 | |
Qatar | 0.28 | ||
Europe | Eastern Europe | Romania | 0.20 |
Bulgaria | 0.19 | ||
Czech Republic | 0.17 | ||
Slovakia | 0.15 | ||
Northern Europe | Lithuania | 0.22 | |
Iceland | 0.18 | ||
Denmark | 0.17 | ||
Southern Europe | Greece | 0.15 | |
Croatia | 0.12 | ||
Oceania | Australia/ New Zealand | Australia | 0.33 |
New Zealand | 0.17 |
19 December 2022–2 January 2023 | ||
Variant | Num seq | Frequency |
22E (Omicron) (BQ.1) | 32 | 0.71 |
22B (Omicron) (BA.5) | 11 | 0.24 |
others | 1 | 0.02 |
23C (Omicron) (CH.1.1) | 1 | 0.02 |
2 January 2023–16 January 2023 | ||
Variant | Num seq | Frequency |
22E (Omicron) (BQ.1) | 26 | 0.72 |
22B (Omicron) (BA.5) | 4 | 0.11 |
22F (Omicron) (XBB) | 2 | 0.06 |
22D (Omicron) (BA.2.75) | 2 | 0.06 |
23C (Omicron) (CH.1.1) | 1 | 0.03 |
21L (Omicron) (BA.2) | 1 | 0.03 |
Africa | 139 | North Africa | 20 |
Eastern Africa | 38 | ||
Middle Africa | 6 | ||
Southern Africa | 52 | ||
Western Africa | 23 | ||
Americas | 881 | Caribbean | 4 |
Central America | 28 | ||
South America | 88 | ||
Northern America | 761 | ||
Asia | 622 | Central Asia | 2 |
Eastern Asia | 184 | ||
South-eastern Asia | 106 | ||
Southern Asia | 117 | ||
Western Asia | 213 | ||
Europe | 992 | Eastern Europe | 32 |
Northern Europe | 338 | ||
Southern Europe | 71 | ||
Western Europe | 551 | ||
Oceania | 19 | Australia/New Zealand | 18 |
Melanesia | 1 |
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Zhakparov, D.; Quirin, Y.; Xiao, Y.; Battaglia, N.; Holzer, M.; Bühler, M.; Kistler, W.; Engel, D.; Zumthor, J.P.; Caduff, A.; et al. Sequencing of SARS-CoV-2 RNA Fragments in Wastewater Detects the Spread of New Variants during Major Events. Microorganisms 2023, 11, 2660. https://doi.org/10.3390/microorganisms11112660
Zhakparov D, Quirin Y, Xiao Y, Battaglia N, Holzer M, Bühler M, Kistler W, Engel D, Zumthor JP, Caduff A, et al. Sequencing of SARS-CoV-2 RNA Fragments in Wastewater Detects the Spread of New Variants during Major Events. Microorganisms. 2023; 11(11):2660. https://doi.org/10.3390/microorganisms11112660
Chicago/Turabian StyleZhakparov, Damir, Yves Quirin, Yi Xiao, Nicole Battaglia, Michael Holzer, Martin Bühler, Walter Kistler, Doortje Engel, Jon Paulin Zumthor, Alexa Caduff, and et al. 2023. "Sequencing of SARS-CoV-2 RNA Fragments in Wastewater Detects the Spread of New Variants during Major Events" Microorganisms 11, no. 11: 2660. https://doi.org/10.3390/microorganisms11112660