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Article

Antimicrobial Susceptibility and Molecular Characterization of Escherichia coli Recovered from Milk and Related Samples

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Department of Animal Science, University for Development Studies, Tamale P.O. Box TL 1882, Ghana
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Department of Food Science and Technology, University for Development Studies, Tamale P.O. Box TL 1882, Ghana
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Department of Veterinary Science, University for Development Studies, Tamale P.O. Box TL 1882, Ghana
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Department of Biotechnology, University for Development Studies, Tamale P.O. Box TL 1882, Ghana
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Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Malaysia
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Author to whom correspondence should be addressed.
Academic Editor: Teresa Semedo-Lemsaddek
Microorganisms 2022, 10(7), 1335; https://doi.org/10.3390/microorganisms10071335
Received: 30 May 2022 / Revised: 20 June 2022 / Accepted: 21 June 2022 / Published: 1 July 2022
(This article belongs to the Special Issue Pathogens and Antimicrobial Drug Resistance in the Food Chain)
There is a rising concern about illnesses resulting from milk consumption due to contamination by pathogenic microorganisms including Escherichia coli. This study examined the occurrence and antimicrobial susceptibility of E. coli isolated from cow milk and related samples. Furthermore, partial sequencing was done to ascertain the genetic relatedness and possible cross contamination among the samples. In all, 250 samples, that is, 50 each of raw milk, cow teat, milkers’ hands, milking utensils, and fecal matter of cows, were cultured for the identification of E. coli. E. coli was detected in 101/250 samples (40.4%). Milk and fecal samples recorded the highest percentages of 68.0% and 66.0%, respectively. Forty-two (42) E. coli strains examined for antimicrobial resistance showed an overall 25.5% resistance, 15.0% intermediate resistance, and 59.5% susceptibility. The isolates had a high level of resistance to teicoplanin (100.0%), but were susceptible to chloramphenicol (95.2%) and azithromycin (92.9%). The Multiple Antibiotic Resistance (MAR) index pattern ranged from 0.1 to 0.5, and 40.5% exhibited multiple drug resistance. The E. coli strains formed 11 haplotypes, and a phylogenic tree analysis showed relatedness among the isolates in other African countries. This observation is an indication of cross contamination among the milk and its related samples. View Full-Text
Keywords: antimicrobial; E. coli; Ghana; partial sequencing; milk; molecular antimicrobial; E. coli; Ghana; partial sequencing; milk; molecular
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MDPI and ACS Style

Adzitey, F.; Yussif, S.; Ayamga, R.; Zuberu, S.; Addy, F.; Adu-Bonsu, G.; Huda, N.; Kobun, R. Antimicrobial Susceptibility and Molecular Characterization of Escherichia coli Recovered from Milk and Related Samples. Microorganisms 2022, 10, 1335. https://doi.org/10.3390/microorganisms10071335

AMA Style

Adzitey F, Yussif S, Ayamga R, Zuberu S, Addy F, Adu-Bonsu G, Huda N, Kobun R. Antimicrobial Susceptibility and Molecular Characterization of Escherichia coli Recovered from Milk and Related Samples. Microorganisms. 2022; 10(7):1335. https://doi.org/10.3390/microorganisms10071335

Chicago/Turabian Style

Adzitey, Frederick, Saniyatu Yussif, Roland Ayamga, Sumaila Zuberu, Francis Addy, Gideon Adu-Bonsu, Nurul Huda, and Rovina Kobun. 2022. "Antimicrobial Susceptibility and Molecular Characterization of Escherichia coli Recovered from Milk and Related Samples" Microorganisms 10, no. 7: 1335. https://doi.org/10.3390/microorganisms10071335

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