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Article

Anti-Melanogenic and Antioxidant Effects of Cell-Free Supernatant from Lactobacillus gasseri BNR17

by 1,2, 1,2,3,4 and 1,2,*
1
AceBiome Inc., Seoul 06164, Korea
2
R&D Center, AceBiome Inc., Daejeon 34013, Korea
3
siRNAgen Therapeutics, Daejeon 34302, Korea
4
Bioneer Corporation, Daejeon 34302, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Iwona Gientka
Microorganisms 2022, 10(4), 788; https://doi.org/10.3390/microorganisms10040788
Received: 3 March 2022 / Revised: 5 April 2022 / Accepted: 7 April 2022 / Published: 8 April 2022
(This article belongs to the Special Issue Advances in Microbial Biosynthesis)
In recent years, there has been considerable interest in the use of cell-free supernatant of probiotics culture for nutritional and functional applications. In this study, we investigated the effect of the cell-free supernatant from Lactobacillus gasseri BNR17 (CFS) on anti-melanogenesis and reducing oxidative stress in B16-F10 murine melanoma cells and HaCaT human keratinocytes. Treatment with CFS significantly inhibited the production of extracellular and intracellular melanin without cytotoxicity during melanogenesis induced by the α-MSH in B16-F10 cells. The CFS dramatically reduced tyrosinase activity and the melanogenesis-related gene expression. Further, it showed antioxidative effects in a dose-dependent manner in DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assays and significantly increased the mRNA levels of HO-1 and CAT in HaCaT cells. Furthermore, the CFS increased HO-1 and anti-oxidative-related gene expression during H2O2-induced oxidative stress in HaCaT cells. Together, this study suggests that the CFS reduces hyperpigmentation and inhibits oxidative stress, and thus can be used as a potential skincare product in the future. View Full-Text
Keywords: cell-free supernatant; Lactobacillus gasseri BNR17; anti-melanogenesis; antioxidative effect cell-free supernatant; Lactobacillus gasseri BNR17; anti-melanogenesis; antioxidative effect
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MDPI and ACS Style

Lee, S.; Park, H.-O.; Yoo, W. Anti-Melanogenic and Antioxidant Effects of Cell-Free Supernatant from Lactobacillus gasseri BNR17. Microorganisms 2022, 10, 788. https://doi.org/10.3390/microorganisms10040788

AMA Style

Lee S, Park H-O, Yoo W. Anti-Melanogenic and Antioxidant Effects of Cell-Free Supernatant from Lactobacillus gasseri BNR17. Microorganisms. 2022; 10(4):788. https://doi.org/10.3390/microorganisms10040788

Chicago/Turabian Style

Lee, Sol, Han-Oh Park, and Wonbeak Yoo. 2022. "Anti-Melanogenic and Antioxidant Effects of Cell-Free Supernatant from Lactobacillus gasseri BNR17" Microorganisms 10, no. 4: 788. https://doi.org/10.3390/microorganisms10040788

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