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Article

Endogenous Honeybee Gut Microbiota Metabolize the Pesticide Clothianidin

1
Department of Biology, Laval University, Québec, QC G1V 0A6, Canada
2
Institut de Biologie Intégrative et des Systèmes (IBIS), Laval University, Québec, QC G1V 0A6, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Michael H. Kogut
Microorganisms 2022, 10(3), 493; https://doi.org/10.3390/microorganisms10030493
Received: 11 January 2022 / Revised: 2 February 2022 / Accepted: 21 February 2022 / Published: 23 February 2022
(This article belongs to the Section Veterinary Microbiology)
Including probiotics in honeybee nutrition represents a promising solution for mitigating diseases, and recent evidence suggests that various microbes possess mechanisms that can bioremediate environmental pollutants. Thus, the use of probiotics capable of degrading pesticides used in modern agriculture would help to both reduce colony losses due to the exposure of foragers to these toxic molecules and improve honeybee health and wellbeing globally. We conducted in vitro experiments to isolate and identify probiotic candidates from bacterial isolates of the honeybee gut (i.e., endogenous strains) according to their ability to (i) grow in contact with three sublethal concentrations of the pesticide clothianidin (0.15, 1 and 10 ppb) and (ii) degrade clothianidin at 0.15 ppb. The isolated bacterial strains were indeed able to grow in contact with the three sublethal concentrations of clothianidin. Bacterial growth rate differed significantly depending on the probiotic candidate and the clothianidin concentration used. Clothianidin was degraded by seven endogenous honeybee gut bacteria, namely Edwardsiella sp., two Serratia sp., Rahnella sp., Pantoea sp., Hafnia sp. and Enterobacter sp., measured within 72 h under in vitro conditions. Our findings highlight that endogenous bacterial strains may constitute the base material from which to develop a promising probiotic strategy to mitigate the toxic effects of clothianidin exposure on honeybee colony health. View Full-Text
Keywords: honeybee; clothianidin; microbiota; probiotic candidate; QuEChERS honeybee; clothianidin; microbiota; probiotic candidate; QuEChERS
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MDPI and ACS Style

El Khoury, S.; Giovenazzo, P.; Derome, N. Endogenous Honeybee Gut Microbiota Metabolize the Pesticide Clothianidin. Microorganisms 2022, 10, 493. https://doi.org/10.3390/microorganisms10030493

AMA Style

El Khoury S, Giovenazzo P, Derome N. Endogenous Honeybee Gut Microbiota Metabolize the Pesticide Clothianidin. Microorganisms. 2022; 10(3):493. https://doi.org/10.3390/microorganisms10030493

Chicago/Turabian Style

El Khoury, Sarah, Pierre Giovenazzo, and Nicolas Derome. 2022. "Endogenous Honeybee Gut Microbiota Metabolize the Pesticide Clothianidin" Microorganisms 10, no. 3: 493. https://doi.org/10.3390/microorganisms10030493

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