Real-Time PCR-Based Methods for Detection of Hepatitis E Virus in Pork Products: A Critical Review
Abstract
:1. Introduction
2. Reverse Transcription Polymerase Chain Amplification-Based Detection of Foodborne Viruses: An Overview of Basic Principles and Controls
3. Real-Time RTPCR-Based Detection Methods for HEV in Pork Products
4. Conclusions
Matrix | Sample Size (g) | Sample Treatment (Prior to NA Extraction) | Reference |
---|---|---|---|
liver | 5–20 g | Blending in PBS | [41] |
liver | 150 mg | Homogenisation by scalpel, bead disruption, proteinase K | [32] |
liver | 0.1 mg | Homogenisation by beating with zirconia beads, lysis reagent, chloroform, centrifugation, gel separation | [56] |
dried and liquid blood products | 200 mg | Mixing with glycine buffer + beef extract | [53] |
figatellu | 10 mg | Fat discarded, homogenisation in PBS, centrifugation | [26] |
liver, kidney, heart | 1 cm3 | As [32] then lysis reagent and chloroform extraction | [36] |
liver, sausage, figatellu | 3 g | Cell disruption in dH2O | [47] |
liver, meat | 10 mg | Bead disruption | [22] |
liver sausage | 3 g | Stomaching in dH2O, centrifugation | [44] |
liver, pate, raw sausages | Homogenisation (ultrasonication?) in Glycine buffer pH9.5, filtration, centrifugation, PEG precipitation, lysis reagent | [55] | |
liver | 1–10 g | As [32] then ultrafiltration | [39] |
salami, boar liver | salami, 5 g; boar liver, 2 g | Stomaching in 7 mL lysis reagent centrifugation, chloroform extraction | [48] |
liver | 10–20 mg | Homogenisation by mortar and pestle | [27] |
liver, chops | liver, 312 mg; chops, 262 mg | Mechanical disruption in lysis buffer, centrifugation | [23] |
Funding
Conflicts of Interest
References
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Cook, N.; D’Agostino, M.; Wood, A.; Scobie, L. Real-Time PCR-Based Methods for Detection of Hepatitis E Virus in Pork Products: A Critical Review. Microorganisms 2022, 10, 428. https://doi.org/10.3390/microorganisms10020428
Cook N, D’Agostino M, Wood A, Scobie L. Real-Time PCR-Based Methods for Detection of Hepatitis E Virus in Pork Products: A Critical Review. Microorganisms. 2022; 10(2):428. https://doi.org/10.3390/microorganisms10020428
Chicago/Turabian StyleCook, Nigel, Martin D’Agostino, Ann Wood, and Linda Scobie. 2022. "Real-Time PCR-Based Methods for Detection of Hepatitis E Virus in Pork Products: A Critical Review" Microorganisms 10, no. 2: 428. https://doi.org/10.3390/microorganisms10020428
APA StyleCook, N., D’Agostino, M., Wood, A., & Scobie, L. (2022). Real-Time PCR-Based Methods for Detection of Hepatitis E Virus in Pork Products: A Critical Review. Microorganisms, 10(2), 428. https://doi.org/10.3390/microorganisms10020428