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Detection of mcr-1 Gene in Undefined Vibrio Species Isolated from Clams

MARE-Marine and Environmental Sciences Centre, ESTM, Polytechnic of Leiria, 2520-630 Peniche, Portugal
iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Associate Laboratory i4HB—Institute for Health and Bioeconomy, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
Author to whom correspondence should be addressed.
These authors contributed equally to the development of this study.
Academic Editor: Nicole Hugouvieux-Cotte-Pattat
Microorganisms 2022, 10(2), 394;
Received: 23 December 2021 / Revised: 24 January 2022 / Accepted: 3 February 2022 / Published: 8 February 2022
(This article belongs to the Section Environmental Microbiology)
The increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic Vibrio are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filter-feeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive Vibrio spp. and 17 coliforms, three Vibrio spp. with MICs to colistin >512 mg L−1 were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the mcr-1 gene. Genotypic analysis for virulence genes in EB07V indicated chiA gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein-coding gene sequences (recA, rpoB, groEL and dnaJ), grouped the isolates into the genus Vibrio but distinguished them as different from any known Vibrio spp. View Full-Text
Keywords: antimicrobial resistance; colistin; mcr-1; Vibrio spp. antimicrobial resistance; colistin; mcr-1; Vibrio spp.
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MDPI and ACS Style

Valdez, C.; Costa, C.; Simões, M.; de Carvalho, C.C.C.R.; Baptista, T.; Campos, M.J. Detection of mcr-1 Gene in Undefined Vibrio Species Isolated from Clams. Microorganisms 2022, 10, 394.

AMA Style

Valdez C, Costa C, Simões M, de Carvalho CCCR, Baptista T, Campos MJ. Detection of mcr-1 Gene in Undefined Vibrio Species Isolated from Clams. Microorganisms. 2022; 10(2):394.

Chicago/Turabian Style

Valdez, Christian, Cátia Costa, Marco Simões, Carla C.C.R. de Carvalho, Teresa Baptista, and Maria J. Campos. 2022. "Detection of mcr-1 Gene in Undefined Vibrio Species Isolated from Clams" Microorganisms 10, no. 2: 394.

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