Microbial Ecology of Artisanal Feta and Kefalograviera Cheeses, Part I: Bacterial Community and Its Functional Characteristics with Focus on Lactic Acid Bacteria as Determined by Culture-Dependent Methods and Phenotype Microarrays
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling
2.2. Physicochemical Characterization
2.3. Microbiological Analyses
2.4. Lactic Acid Microbiota Identification
2.4.1. Classical Identification
2.4.2. Molecular Identification
2.4.3. Biolog Microbial Identification with GEN III Microplates
3. Results
3.1. Physicochemical and Microbiological Characterization
3.2. Lactic Acid Microbiota Identification
3.2.1. Classical Identification
3.2.2. Molecular Identification
3.2.3. Biolog Microbial Identification with GEN III Microplates
3.3. Lactic Acid Bacteria Microecosystem of Raw Sheep’s Milk, Feta and Kefalograviera
3.4. Biolog Microbial Community Assay with EcoPlates
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Samples | Fat (%) | Moisture (%) | pH | NaCl (%) | Lactose (%) | Protein (%) | Fat Hydrolysis | Protein Hydrolysis | MRS Counts (log CFU/mL) |
---|---|---|---|---|---|---|---|---|---|
Feta | |||||||||
Early ripening | 25.5 | 51.3 | 5.07 | 2.53 | 0 | 15.85 | 3.9 | 0.764 | 7.02 |
Late ripening | 26 | 49.3 | 4.25 | 3.4 | 0 | 16.24 | 2 | 0.702 | 6.60 |
Kefalograviera | |||||||||
Early ripening | 30.5 | 36.1 | 5.14 | 3.05 | 0.3 | 26.28 | 2.5 | 0.58 | 6.02 |
Late ripening | 30 | 36.7 | 5.12 | 2.91 | 0.31 | 26.10 | 4.1 | 0.589 | 6.77 |
Cheese Samples | Richness—S | Shannon-Wiener—H | Shannon Evenness—E | Simpson—D |
---|---|---|---|---|
Feta-ER | 2.500 ± 0.236 a | 1.154 ± 0.104 a | 1.349 ± 0.319 a | 0.601 ± 0.012 a |
Feta-LR | 8.667 ± 0.472 b | 2.307 ± 0.040 b | 1.069 ± 0.008 a | 0.880 ± 0.006 a |
Kefalograviera-ER | 14.167 ± 0.235 c | 2.650 ± 0.003 b,c | 1.002 ± 0.006 a | 0.922 ± 0.001 a |
Kefalograviera-LR | 7.167 ± 1.179 d | 1.821 ± 0.173 d | 0.932 ± 0.003 a | 0.810 ± 0.039 a |
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Tsigkrimani, M.; Bakogianni, M.; Paramithiotis, S.; Bosnea, L.; Pappa, E.; Drosinos, E.H.; Skandamis, P.N.; Mataragas, M. Microbial Ecology of Artisanal Feta and Kefalograviera Cheeses, Part I: Bacterial Community and Its Functional Characteristics with Focus on Lactic Acid Bacteria as Determined by Culture-Dependent Methods and Phenotype Microarrays. Microorganisms 2022, 10, 161. https://doi.org/10.3390/microorganisms10010161
Tsigkrimani M, Bakogianni M, Paramithiotis S, Bosnea L, Pappa E, Drosinos EH, Skandamis PN, Mataragas M. Microbial Ecology of Artisanal Feta and Kefalograviera Cheeses, Part I: Bacterial Community and Its Functional Characteristics with Focus on Lactic Acid Bacteria as Determined by Culture-Dependent Methods and Phenotype Microarrays. Microorganisms. 2022; 10(1):161. https://doi.org/10.3390/microorganisms10010161
Chicago/Turabian StyleTsigkrimani, Markella, Magdalini Bakogianni, Spiros Paramithiotis, Loulouda Bosnea, Eleni Pappa, Eleftherios H. Drosinos, Panagiotis N. Skandamis, and Marios Mataragas. 2022. "Microbial Ecology of Artisanal Feta and Kefalograviera Cheeses, Part I: Bacterial Community and Its Functional Characteristics with Focus on Lactic Acid Bacteria as Determined by Culture-Dependent Methods and Phenotype Microarrays" Microorganisms 10, no. 1: 161. https://doi.org/10.3390/microorganisms10010161
APA StyleTsigkrimani, M., Bakogianni, M., Paramithiotis, S., Bosnea, L., Pappa, E., Drosinos, E. H., Skandamis, P. N., & Mataragas, M. (2022). Microbial Ecology of Artisanal Feta and Kefalograviera Cheeses, Part I: Bacterial Community and Its Functional Characteristics with Focus on Lactic Acid Bacteria as Determined by Culture-Dependent Methods and Phenotype Microarrays. Microorganisms, 10(1), 161. https://doi.org/10.3390/microorganisms10010161