Does Curcumin Have a Role in the Interaction between Gut Microbiota and Schistosoma mansoni in Mice?
Abstract
:1. Introduction
2. Results
2.1. Impact of S. Mansoni Infection on the Composition of Gut Microbiota
2.2. Influence of Curcumin on the Composition of Mice Gut Microbiota
2.3. Schistosomes, CUR, and Gut Microbiota Interactions
2.3.1. Effects on the Microbiota
2.3.2. Effects on Immune Responses against Schistosome Egg Antigens
2.3.3. Effects on Parasitological Parameters
3. Discussion
4. Materials and Methods
4.1. Curcumin
4.2. Animals
4.3. Ethical Consideration
4.4. Experimental Design
4.5. Bleeding and Sample Collection
4.6. Microbiological Parameters
4.7. Immunological Parameters
4.7.1. Antibody Assay
4.7.2. Cytokines Assay
4.8. Parasitological Parameters
4.8.1. Worm Burden
4.8.2. Oogram Map
4.8.3. Total Egg Counts
4.8.4. Egg Hatchability
4.9. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Groups | Mean ± SD | |||||
---|---|---|---|---|---|---|
IgM | IgG1 | IgG2a | IgG2b | IgA | IgE | |
Naïve | 0.089 ± 0.010 | 0.032 ± 0.017 | 0.066 ± 0.033 | 0.069 ± 0.008 | 0.037 ± 0.045 | 0.004 ± 0.002 |
(Cut off) | (0.109) | (0.066) | (0.132) | (0.085) | (0.127) | (0.008) |
I | 0.284 ± 0.038 * | 0.180 ± 0.024 * | 0.063 ± 0.018 | 0.070 ± 0.017 | 0.045 ± 0.013 | 0.006 ± 0.005 |
ID | 0.219 ± 0.067 * | 0.112 ± 0.076 * | 0.049 ± 0.036 | 0.138 ± 0.045 * | 0.143 ± 0.019 * | 0.007 ± 0.003 |
IL | 0.289 ± 0.101 * | 0.189 ± 0.056 * | 0.041 ± 0.021 | 0.053 ± 0.034 | 0.056 ± 0.025 | 0.007 ± 0.003 |
IH | 0.274 ± 0.075 * | 0.598 ± 0.130 * | 0.036 ± 0.033 | 0.062 ± 0.020 | 0.120 ± 0.040 | 0.007 ± 0.003 |
Parameter Counts | I | ID | IL | IH |
---|---|---|---|---|
Total worm burden | ||||
Mean ± SD | 43.34 ± 8.44 | 42.0 ± 2.65 | 42.0 ± 15.25 | 15.60 ± 10.01 |
p value | 0.81 | 0.9 | 0.007 *** | |
Reduction (%) | 3.10% | 3.10% | 64.00% | |
Male worm burden | ||||
Mean ± SD | 24.67 ± 3.95 | 23.0 ± 3.61 | 26.25 ± 11.15 | 8.40 ± 5.90 |
p value | 0.62 | 0.83 | 0.006 *** | |
Reduction (%) | 6.70% | 65.90% | ||
Female worm burden | ||||
Mean ± SD | 18.67 ± 4.46 | 19.0 ± 4.58 | 15.75 ± 5.12 | 7.20 ± 4.87 |
p value | 0.93 | 0.47 | 0.005 *** | |
Reduction (%) | 77.70% | 15.60% | 61.40% | |
Liver egg counts | ||||
Mean ± SD | 40333 ± 17616 | 48333.3 ± 14433.76 | 63750 ± 17100.2 | 48333 ± 22317 |
p value | 0.58 | 0.14 | 0.61 | |
Reduction (%) | - | - | ||
Intestine egg counts | ||||
Mean ± SD | 68500 ± 2121.3 | 52750 ± 30774.7 | 53500 ± 33426.5 | 39666 ± 14982.2 |
p value | 0.53 | 0.58 | 0.042 *** | |
Reduction (%) | 21.90% | 42.10% | ||
% Immature ova | ||||
Mean ± SD | 33.70 ± 7.42 | 35.48 ± 12.5 | 24.9 ± 6.1 | 20.86 ± 5.22 |
p value | 0.84 | 0.19 | 0.02 *** | |
Reduction (%) | 27.30% | 39.40% | ||
% Mature ova | ||||
Mean ± SD | 58.38 ± 8.85 | 57.57 ± 11.03 | 62.97 ± 5.2 | 50.25 ± 11.5 |
p value | 0.92 | 0.48 | 0.32 | |
Reduction (%) | 1.70% | 13.70% | ||
% Dead ova | ||||
Mean ± SD | 7.83 ± 4.51 | 6.96 ± 3.0 | 12.13 ± 4.6 | 28.9 ± 10.9 |
p value | 0.7 | 0.22 | 0.02 *** | |
Increase (%) | 34.90% | 72.90% |
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Anter, A.; El-Ghany, M.A.; Abou El Dahab, M.; Mahana, N. Does Curcumin Have a Role in the Interaction between Gut Microbiota and Schistosoma mansoni in Mice? Pathogens 2020, 9, 767. https://doi.org/10.3390/pathogens9090767
Anter A, El-Ghany MA, Abou El Dahab M, Mahana N. Does Curcumin Have a Role in the Interaction between Gut Microbiota and Schistosoma mansoni in Mice? Pathogens. 2020; 9(9):767. https://doi.org/10.3390/pathogens9090767
Chicago/Turabian StyleAnter, Assmaa, Mohamed Abd El-Ghany, Marwa Abou El Dahab, and Noha Mahana. 2020. "Does Curcumin Have a Role in the Interaction between Gut Microbiota and Schistosoma mansoni in Mice?" Pathogens 9, no. 9: 767. https://doi.org/10.3390/pathogens9090767