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Article

Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection

1
Department of Virology, Veterinary Research Institute, 621 00 Brno, Czech Republic
2
Fish Disease Research Unit, Institute for Parasitology, University of Veterinary Medicine, 30559 Hannover, Germany
3
Department of Ecology and Diseases of Zooanimals, Game, Fish and Bees, Veterinary and Pharmaceutical University, 612 42 Brno, Czech Republic
4
Department of Immunology, Veterinary Research Institute, 621 00 Brno, Czech Republic
5
Department of Zoology, Fisheries, Hydrobiology and Apiculture, Mendel University, 613 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(8), 604; https://doi.org/10.3390/pathogens9080604
Received: 30 June 2020 / Revised: 17 July 2020 / Accepted: 21 July 2020 / Published: 24 July 2020
The population of brown trout (Salmo trutta fario) in continental Europe is on the decline, with infectious diseases confirmed as one of the causative factors. However, no data on the epizootiological situation of wild fish in the Czech Republic are currently available. In this study, brown trout (n = 260) from eight rivers were examined for the presence of viral and parasitical pathogens. Salmonid alphavirus-2, infectious pancreatic necrosis virus, piscine novirhabdovirus (VHSV) and salmonid novirhabdovirus (IHNV) were not detected using PCR. Cell culturing showed no viruses as well, and serological analysis of 110 sera did not detect any specific antibodies against VHSV or IHNV. Fish from two rivers were positive for the presence of piscine orthoreovirus-3 (PRV-3), subtype PRV-3b. However, none of the PRV-3-positive fish showed gross pathologies typically associated with PRV infections. By far the most widespread pathogen was Tetracapsuloides bryosalmonae which was confirmed in each of the examined locations, with a prevalence of up to 65% and 100%, as established by immunohistochemistry and PCR, respectively. Furthermore, up to 43.8% of fish showed signs of proliferative kidney disease caused by T. bryosalmonae, suggesting that this parasite is a main health challenge for brown trout in the Czech Republic. View Full-Text
Keywords: immunohistochemistry; infectious hematopoietic necrosis; infectious pancreatic necrosis; PCR; salmonid alphavirus; serology; Tetracapsuloides bryosalmonae; viral hemorrhagic septicemia immunohistochemistry; infectious hematopoietic necrosis; infectious pancreatic necrosis; PCR; salmonid alphavirus; serology; Tetracapsuloides bryosalmonae; viral hemorrhagic septicemia
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MDPI and ACS Style

Pojezdal, Ľ.; Adamek, M.; Syrová, E.; Steinhagen, D.; Minářová, H.; Papežíková, I.; Seidlová, V.; Reschová, S.; Palíková, M. Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection. Pathogens 2020, 9, 604. https://doi.org/10.3390/pathogens9080604

AMA Style

Pojezdal Ľ, Adamek M, Syrová E, Steinhagen D, Minářová H, Papežíková I, Seidlová V, Reschová S, Palíková M. Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection. Pathogens. 2020; 9(8):604. https://doi.org/10.3390/pathogens9080604

Chicago/Turabian Style

Pojezdal, Ľubomír, Mikolaj Adamek, Eva Syrová, Dieter Steinhagen, Hana Minářová, Ivana Papežíková, Veronika Seidlová, Stanislava Reschová, and Miroslava Palíková. 2020. "Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection" Pathogens 9, no. 8: 604. https://doi.org/10.3390/pathogens9080604

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