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Open AccessArticle

Cold Exposure-Induced Up-Regulation of Hsp70 Positively Regulates PEDV mRNA Synthesis and Protein Expression In Vitro

by Fanzhi Kong 1,2,†, Yaru Xu 2,†, Wei Ran 2, Baishuang Yin 3, Li Feng 1,* and Dongbo Sun 2,*
1
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
2
Laboratory for the Prevention and Control of Swine Infectious Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing 163319, China
3
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 132101 Jilin, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Pathogens 2020, 9(4), 246; https://doi.org/10.3390/pathogens9040246
Received: 30 January 2020 / Revised: 14 March 2020 / Accepted: 24 March 2020 / Published: 26 March 2020
(This article belongs to the Section Animal Pathogens)
Porcine epidemic diarrhea (PED) is a highly contagious, intestinal infectious disease caused by porcine epidemic diarrhea virus (PEDV). PEDV as an emerging and re-emerging epizootic virus of swine causes substantial economic losses to the pig industry in China and other countries. In China, the occurrence of PED shows significant seasonal variations, usually outbreak during the winter season. The epidemic characteristics of PED may be highly correlated with the changes of ambient temperature. However, molecular mechanism on the seasonal occurrence of PED still remains unclear. It has been widely observed that low ambient temperature up-regulates the expression of host heat shock protein 70 (Hsp70). Here, we showed that nucleotide and protein levels of Hsp70 were up-regulated in the intestinal of cold exposed pig and cold exposed Vero E6 cells. We found that overexpression of Hsp70 could increase PEDV mRNA synthesis and protein expression in Vero E6 and IPEC-J2 cells, while the siRNAs mediated knockdown of Hsp70 and VER155008 mediated inhibition of Hsp70 resulted in inhibition of viral mRNA synthesis and protein expression in Vero E6 cells. These data suggested that Hsp70 positively regulated PEDV mRNA synthesis and protein expression, which being helpful for understanding the seasonality of PED epidemics and development of novel antiviral therapies in the future. View Full-Text
Keywords: cold exposure; Hsp70; porcine epidemic diarrhea virus; viral mRNA synthesis and protein expression cold exposure; Hsp70; porcine epidemic diarrhea virus; viral mRNA synthesis and protein expression
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Kong, F.; Xu, Y.; Ran, W.; Yin, B.; Feng, L.; Sun, D. Cold Exposure-Induced Up-Regulation of Hsp70 Positively Regulates PEDV mRNA Synthesis and Protein Expression In Vitro. Pathogens 2020, 9, 246.

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