Country-Wide qPCR Based Assessment of Plasmodiophora brassicae Spread in Agricultural Soils and Recommendations for the Cultivation of Brassicaceae Crops in Poland
Abstract
:1. Introduction
2. Results
2.1. Establishment of the Linear Regression between Microscope Observations and qPCR Results
2.2. Quantification of P. brassicae in Soil Samples Using Real-Time qPCR
3. Discussion
4. Material and Methods
4.1. Soil Sampling
4.2. DNA Extraction from Soil
4.3. Real-Time qPCR
4.4. Quantification of Plasmodiophora Brassicae Spores
4.5. Cultivation Guidelines Based on qPCR Assay
4.6. Preparation of Maps
4.7. Statistical Analysis and Data Integration
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Czubatka-Bieńkowska, A.; Kaczmarek, J.; Marzec-Schmidt, K.; Nieróbca, A.; Czajka, A.; Jędryczka, M. Country-Wide qPCR Based Assessment of Plasmodiophora brassicae Spread in Agricultural Soils and Recommendations for the Cultivation of Brassicaceae Crops in Poland. Pathogens 2020, 9, 1070. https://doi.org/10.3390/pathogens9121070
Czubatka-Bieńkowska A, Kaczmarek J, Marzec-Schmidt K, Nieróbca A, Czajka A, Jędryczka M. Country-Wide qPCR Based Assessment of Plasmodiophora brassicae Spread in Agricultural Soils and Recommendations for the Cultivation of Brassicaceae Crops in Poland. Pathogens. 2020; 9(12):1070. https://doi.org/10.3390/pathogens9121070
Chicago/Turabian StyleCzubatka-Bieńkowska, Anna, Joanna Kaczmarek, Katarzyna Marzec-Schmidt, Anna Nieróbca, Agnieszka Czajka, and Małgorzata Jędryczka. 2020. "Country-Wide qPCR Based Assessment of Plasmodiophora brassicae Spread in Agricultural Soils and Recommendations for the Cultivation of Brassicaceae Crops in Poland" Pathogens 9, no. 12: 1070. https://doi.org/10.3390/pathogens9121070