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Relation between Biofilm and Virulence in Vibrio tapetis: A Transcriptomic Study

1
Laboratoire de Biotechnologie et Chimie Marines (LBCM), EA 3884, LBCM, IUEM Université de Bretagne-Sud, 56100 Lorient, France
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UMR6539, Laboratoire des Sciences de l’Environnement Marin (LEMAR), Centre National de la Recherche Scientifique, Institut Universitaire Européen de la Mer, Université de Brest, UBO, IRD, Ifremer, 29280 Plouzané, France
3
DuPont Nutrition and Health, Danisco France SAS, BP10, F-86220 Dangé-Saint-Romain, France
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Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, University Rouen-Normandy, 27000 Evreux, France
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UMR 7267, Laboratoire d’Ecologie et Biologie des interactions (EBI), Université de Poitiers, 86000 Poitiers, France
*
Author to whom correspondence should be addressed.
Present Address: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, University of Rouen, 27000 Evreux, France.
Pathogens 2018, 7(4), 92; https://doi.org/10.3390/pathogens7040092
Received: 28 September 2018 / Revised: 20 November 2018 / Accepted: 23 November 2018 / Published: 26 November 2018
Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. Vibrio tapetis can develop such biofilms on the inner surface of shells of the Ruditapes philippinarum clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of V. tapetis is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. In the present study, we used RNA-seq analysis to identify biofilm- and virulence-related genes displaying altered expression in biofilms compared to the planktonic condition. A flow cell system was employed to grow biofilms to obtain both structural and transcriptomic views of the biofilms. We found that 3615 genes were differentially expressed, confirming that biofilm and planktonic lifestyles are very different. As expected, the differentially expressed genes included those involved in biofilm formation, such as motility- and polysaccharide synthesis-related genes. The data show that quorum sensing is probably mediated by the AI-2/LuxO system in V. tapetis biofilms. The expression of genes encoding the Type VI Secretion System and associated exported proteins are strongly induced, suggesting that V. tapetis activates this virulence factor when living in biofilm. View Full-Text
Keywords: biofilm; virulence; Vibrio tapetis; transcriptome; quorum sensing; type VI secretion system; brown ring disease biofilm; virulence; Vibrio tapetis; transcriptome; quorum sensing; type VI secretion system; brown ring disease
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Rodrigues, S.; Paillard, C.; Van Dillen, S.; Tahrioui, A.; Berjeaud, J.-M.; Dufour, A.; Bazire, A. Relation between Biofilm and Virulence in Vibrio tapetis: A Transcriptomic Study. Pathogens 2018, 7, 92.

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