Next Article in Journal
Pharmacological Agents Targeting the Cellular Prion Protein
Previous Article in Journal
Congenital Toxoplasmosis: A Plea for a Neglected Disease
Previous Article in Special Issue
Bioenergetics of Mycobacterium: An Emerging Landscape for Drug Discovery
Open AccessFeature PaperArticle

Soluble CD14 as a Diagnostic Biomarker for Smear-Negative HIV-Associated Tuberculosis

1
Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USA
2
Africa Health Research Institute, Durban 4013, South Africa
3
Department of Epidemiology and Population Health, Albert Einstein College of Medicine, New York, NY 10461, USA
4
HIV Pathogenesis Programme, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal, Durban 4013, South Africa
5
Ragon Institute of MGH, MIT and Harvard University, Cambridge, MA 02139, USA
6
Max Planck Institute of Infection Biology, 10117 Berlin, Germany
7
Department of Medicine, Edendale Hospital, University of KwaZulu-Natal, Pietermaritzburg 3201, South Africa
8
Department of Microbiology and Immunology, Albert Einstein College of Medicine, New York, NY 10461, USA
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Pathogens 2018, 7(1), 26; https://doi.org/10.3390/pathogens7010026
Received: 25 January 2018 / Revised: 20 February 2018 / Accepted: 21 February 2018 / Published: 27 February 2018
(This article belongs to the Special Issue Mechanisms of Mycobacterium tuberculosis Pathogenesis)
Sputum smear-negative HIV-associated active tuberculosis (TB) is challenging to diagnose. CD14 is a pattern recognition receptor that is known to mediate monocyte activation. Prior studies have shown increased levels of soluble CD14 (sCD14) as a potential biomarker for TB, but little is known about its value in detecting smear-negative HIV-associated TB. We optimized a sandwich ELISA for the detection of sCD14, and tested sera from 56 smear-negative South African (39 culture-positive and 17 culture-negative) HIV-infected pulmonary TB patients and 24 South African and 43 US (21 positive and 22 negative for tuberculin skin test, respectively) HIV-infected controls. SCD14 concentrations were significantly elevated in smear-negative HIV-associated TB compared with the HIV-infected controls (p < 0.0001), who had similar concentrations, irrespective of the country of origin or the presence or absence of latent M. tuberculosis infection (p = 0.19). The culture-confirmed TB group had a median sCD14 level of 2199 ng/mL (interquartile range 1927–2719 ng/mL), versus 1148 ng/mL (interquartile range 1053–1412 ng/mL) for the South African controls. At a specificity of 96%, sCD14 had a sensitivity of 95% for culture-confirmed smear-negative TB. These data indicate that sCD14 could be a highly accurate biomarker for the detection of HIV-associated TB. View Full-Text
Keywords: tuberculosis; biomarker; diagnostics; HIV; CD14; C-reactive protein tuberculosis; biomarker; diagnostics; HIV; CD14; C-reactive protein
Show Figures

Figure 1

MDPI and ACS Style

Liu, Y.; Ndumnego, O.C.; Chen, T.; Kim, R.S.; Jenny-Avital, E.R.; Ndung’u, T.; Wilson, D.; Achkar, J.M. Soluble CD14 as a Diagnostic Biomarker for Smear-Negative HIV-Associated Tuberculosis. Pathogens 2018, 7, 26.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop