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Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP

Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, NUHS Tower Block, Level 10, Singapore 119074, Singapore
Infectious Diseases Group, Genome Institute of Singapore, 60 Biopolis street, Genome, #02-01, Singapore 138672, Singapore
Author to whom correspondence should be addressed.
Academic Editor: Catharina Svanborg
Pathogens 2016, 5(1), 3;
Received: 11 September 2015 / Revised: 2 December 2015 / Accepted: 29 December 2015 / Published: 5 January 2016
(This article belongs to the Special Issue Molecular Aspects of Urinary Tract Infection)
Fluorescent proteins, especially green fluorescent protein (GFP), have been instrumental in understanding urinary tract infection pathogenesis by uropathogenic Escherichia coli (UPEC). We have used a recently developed GFP variant, vsfGFP-9, to create new plasmid- and chromosome-based GFP derivatives of the UPEC strain UTI89. The vsfGFP-9 strains are nearly 10× brighter with no in vitro growth or in vivo virulence defects compared to previously reported GFP-expressing UTI89 strains. The chromosomal vsfGFP-9 strain is equivalent to the wild type UTI89 during in vivo UTI, while both plasmid GFP constructs have an equivalent virulence defect compared to non-plasmid carrying UTI89. These new vsfGFP-9 expressing strains should be useful for further studies of the pathogenesis of UTI89, and similar strategies can be used to create improved fluorescent derivatives of other UPEC strains. View Full-Text
Keywords: GFP; uropathogenic Escherichia coli; urinary tract infection GFP; uropathogenic Escherichia coli; urinary tract infection
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Eshaghi, M.; Mehershahi, K.S.; Chen, S.L. Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP. Pathogens 2016, 5, 3.

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