Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress

Lin, Chi-Fen; Yu, Kun-Hua; Jheng, Cheng-Ping; Chung, Raymond; Lee, Cheng-I

doi:10.3390/pathogens2030506

Volume 2, Issue 3

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Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress

Chi-Fen Lin^1,2,†, Kun-Hua Yu^1,†, Cheng-Ping Jheng¹, Raymond Chung^3,* and Cheng-I Lee^1,*

Department of Life Science, Institute of Molecular Biology and Institute of Biomedical Science, College of Science, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan 621, China

Department of Clinical Pathology, Buddhist Dalin Tzu Chi General Hospital, Chia-Yi, Taiwan 622, China

Department of Chemistry and Biochemistry, Manhattan College, Riverdale, NY 10471, USA

Authors to whom correspondence should be addressed.

^†

Two authors contributed equally to this work.

Pathogens 2013, 2(3), 506-519; https://doi.org/10.3390/pathogens2030506

Received: 10 July 2013 / Revised: 17 July 2013 / Accepted: 21 July 2013 / Published: 25 July 2013

(This article belongs to the Special Issue Prions)

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Abstract

Misfolding and aggregation into amyloids of the prion protein (PrP) is responsible for the development of fatal transmissible neurodegenerative diseases. Various studies on curcumin demonstrate promise for the prevention of Alzheimer’s disease and inhibition of PrP^res accumulation. To evaluate the effect of curcumin on amyloid fibrillation of prion protein, we first investigated the effect of curcumin on mouse prion protein (mPrP) in a cell-free system. Curcumin reduced the prion fibril formation significantly. Furthermore, we monitored the change in apoptosis and reactive oxygen species (ROS) level upon curcumin treatment in mouse neuroblastoma cells (N2a). Curcumin effectively rescues the cells from apoptosis and decreases the ROS level caused by subsequent co-incubation with prion amyloid fibrils. The assays in cell-free mPrP and in N2a cells of this work verified the promising effect of curcumin on the prevention of transmissible neurodegenerative diseases. View Full-Text

Keywords: prion; amyloid; fibril; curcumin; apoptosis; ROS

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Lin, C.-F.; Yu, K.-H.; Jheng, C.-P.; Chung, R.; Lee, C.-I. Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress. Pathogens 2013, 2, 506-519.