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Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus

1
National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan
2
Tokyo University of Agriculture and Technology, 3-5-8 Fuchu, Tokyo, 183-8509, Japan
3
Japan Anti-tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo, 204-8533, Japan
4
Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan
5
National Institute of Biomedical Innovation, 1 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan
6
Primate Research Institute, Kyoto University, Inuyama, Aichi, 484-8506, Japan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work.
Pathogens 2013, 2(2), 357-363; https://doi.org/10.3390/pathogens2020357
Received: 25 March 2013 / Revised: 27 April 2013 / Accepted: 8 May 2013 / Published: 13 May 2013
(This article belongs to the Special Issue Pathogen Infection Models)
Common marmosets (Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the present study. Levels of DENV genome were determined in 228 urine samples from 20 primary DENV-inoculated marmosets and in 56 urine samples from four secondary DENV-inoculated marmosets. DENV genome was detected in 75% (15/20) of marmosets after primary DENV infection. No DENV genome was detected in urine samples from the marmosets with secondary infection with homologous DENV (0%, 0/4). Two marmosets demonstrated hematuria. Pathological analysis of the kidneys demonstrated non-suppressive interstitial nephritis with renal tubular regeneration. DENV antigen-positive cells were detected in kidneys. In human dengue virus infections, some patients present renal symptoms. The results indicate that marmosets recapitulate some aspects of the involvement of kidneys in human DENV infection, and suggest that marmosets are potentially useful for the studies of the pathogenesis of DENV infection, including kidneys. View Full-Text
Keywords: dengue virus; animal model; marmoset; urine; hematuria; kidney dengue virus; animal model; marmoset; urine; hematuria; kidney
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Moi, M.L.; Omatsu, T.; Hirayama, T.; Nakamura, S.; Katakai, Y.; Yoshida, T.; Saito, A.; Tajima, S.; Ito, M.; Takasaki, T.; Akari, H.; Kurane, I. Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus. Pathogens 2013, 2, 357-363.

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