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Pathogens
Volume 14
Issue 9
10.3390/pathogens14090861
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Article

Microbiological and Clinical Short-Term Evaluation of the Efficacy of an Herbal Tincture as an Adjunctive Treatment in the Management of Stage II, Grade A Periodontitis

by
Milica Petrović
1,*,
Ljiljana Kesić
1,
Bojana Miladinović
2,
Radmila Obradović
1,
Ana Pejčić
1,
Marija Bojović
1,
Katarina Šavikin
3,
Jelena Živković
3,
Ivana Stanković
1 and
Dušanka Kitić
2,*
1
Department of Oral Medicine and Periodontology, Dental Clinic, Faculty of Medicine, University of Niš, 18108 Niš, Serbia
2
Department of Pharmacy, Faculty of Medicine, University of Niš, 18108 Niš, Serbia
3
Institute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
*
Authors to whom correspondence should be addressed.
Pathogens 2025, 14(9), 861; https://doi.org/10.3390/pathogens14090861
Submission received: 19 June 2025 / Revised: 24 August 2025 / Accepted: 28 August 2025 / Published: 29 August 2025
(This article belongs to the Special Issue Oral Microbiome and Human Systemic Health)
Versions Notes

Abstract

The increased incidence of periodontitis, the resistance of periodontal pathogens to antibiotics, and the adverse effects of certain drugs used in general dentistry present a strong rationale for seeking safe and effective plant-based treatments for periodontitis. HPLC-DAD analysis of a commercial herbal tincture confirmed the presence of rosmarinic acid (1102.79 ± 21.56 µg/mL), luteolin-7-O-glucoside (358.06 ± 5.64 µg/mL), and isorhamnetin (24.17 ± 0.49 µg/mL), bioactive phytochemicals known for their antimicrobial and anti-inflammatoryproperties. The randomized prospective study analyzed Tinctura paradentoica® as an adjunct to anti-infectious non-surgical periodontal therapy (NSPT) on clinical and microbiological parameters in patients with moderate periodontitis (Stage II, Grade A). All 60 recruited participants were randomly allocated to either the intervention group (NSPT + Tinctura paradentoica®) or the control group (NSPT alone). The rate of prevalence of the following periodontopathogenic microorganisms (Treponema denticola, Tannerella forsythensis), assessed by polymerase chain reaction (PCR) analysis, was significantly lower in the intervention group (p < 0.001), but no statistically significant difference was found for Porphyromonas gingivalis. The herbal tincture, combined with NSPT, produces a short-term reduction in periodontal clinical parameters (Green–Vermilion plaque index, bleeding on probing index (BOP), and clinical attachment level (CAL), without clinical relevance, and the prevalence of the following bacteria species (Tannerella forsythensis, Treponema denticola).
Keywords: periodontal disease; anaerobic bacteria; herbal remedies; polymerase chain reaction periodontal disease; anaerobic bacteria; herbal remedies; polymerase chain reaction

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MDPI and ACS Style

Petrović, M.; Kesić, L.; Miladinović, B.; Obradović, R.; Pejčić, A.; Bojović, M.; Šavikin, K.; Živković, J.; Stanković, I.; Kitić, D. Microbiological and Clinical Short-Term Evaluation of the Efficacy of an Herbal Tincture as an Adjunctive Treatment in the Management of Stage II, Grade A Periodontitis. Pathogens 2025, 14, 861. https://doi.org/10.3390/pathogens14090861

AMA Style

Petrović M, Kesić L, Miladinović B, Obradović R, Pejčić A, Bojović M, Šavikin K, Živković J, Stanković I, Kitić D. Microbiological and Clinical Short-Term Evaluation of the Efficacy of an Herbal Tincture as an Adjunctive Treatment in the Management of Stage II, Grade A Periodontitis. Pathogens. 2025; 14(9):861. https://doi.org/10.3390/pathogens14090861

Chicago/Turabian Style

Petrović, Milica, Ljiljana Kesić, Bojana Miladinović, Radmila Obradović, Ana Pejčić, Marija Bojović, Katarina Šavikin, Jelena Živković, Ivana Stanković, and Dušanka Kitić. 2025. "Microbiological and Clinical Short-Term Evaluation of the Efficacy of an Herbal Tincture as an Adjunctive Treatment in the Management of Stage II, Grade A Periodontitis" Pathogens 14, no. 9: 861. https://doi.org/10.3390/pathogens14090861

APA Style

Petrović, M., Kesić, L., Miladinović, B., Obradović, R., Pejčić, A., Bojović, M., Šavikin, K., Živković, J., Stanković, I., & Kitić, D. (2025). Microbiological and Clinical Short-Term Evaluation of the Efficacy of an Herbal Tincture as an Adjunctive Treatment in the Management of Stage II, Grade A Periodontitis. Pathogens, 14(9), 861. https://doi.org/10.3390/pathogens14090861

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