Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil
Abstract
:1. Introduction
2. Materials and Methods
2.1. Canine Serum Sample
2.2. Clinical Examinations
2.3. Serological Tests
2.4. Statistical Analyses
3. Results
3.1. Comparative Testing of IFAT, ELISA and LFT for CanL Serodiagnosis in Croatia
3.2. Evaluation of the rKLi8.3 ELISA and LFT in Healthy, Vaccinated and Infected Dogs from Brazil
3.3. Detection of CanL-Specific IgG Antibodies in Dogs from Croatia and Brazil by rKLi8.3 Lineblot
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dogs (n) | In house IFAT | VetLine® ELISA | rKLi8.3 ELISA | LFT INgezim® | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Pos | Neg | Pos | Neg | Pos | Neg | Pos | Neg | |||||
Symptomatic (66) | 63 | 3 | Sensitivity 95.4% | 58 | 8 | Sensitivity 87.8% | 64 | 2 | Sensitivity 96.9% | 64 | 2 | Sensitivity 96.9% |
Asymptomatic (27) | 24 | 3 | Sensitivity 88.8% | 19 | 8 | Sensitivity 70.3% | 23 | 4 | Sensitivity 85.1% | 22 | 5 | Sensitivity 81.4% |
Healthy endemic (88) | 1 | 87 | Specificity 98.8% | 13 | 73 | Specificity 85.2% | 1 | 87 | Specificity 98.8% | 2 | 86 | Specificity 97.7% |
Other infections (51) | 0 | 51 | Specificity 100% | 2 | 49 | Specificity 96% | 0 | 51 | Specificity 100% | 0 | 51 | Specificity 100% |
Dogs (n) | VetBlot Leishmania® LineBlot | VetLine® ELISA | rKLi8.3 ELISA | LFT INgezim® | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Pos | Neg | Pos | Neg | Pos | Neg | Pos | Neg | |||||
Symptomatic (24) | 24 | 0 | Sensitivity 100% | 24 | 0 | Sensitivity 100% | 24 | 0 | Sensitivity 100% | 24 | 0 | Sensitivity 100% |
Asymptomatic (15) | 12 | 3 | Sensitivity 80% | 10 | 5 | Sensitivity 66.6% | 13 | 2 | Sensitivity 86.6% | 13 | 2 | Sensitivity 86.6% |
Healthy endemic (52) | 3 | 49 | Specificity 94.2% | 7 | 45 | Specificity 86.5% | 0 | 52 | Specificity 100% | 1 | 51 | Specificity 98% |
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Mahdavi, R.; Martinkovic, F.; Shams-Eldin, H.; Pereira, I.E.; Reis, A.B.; Latz, A.; Heinz, D.; Aira, C.; Fresco-Taboada, A.; Abass, E.; et al. Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil. Pathogens 2024, 13, 109. https://doi.org/10.3390/pathogens13020109
Mahdavi R, Martinkovic F, Shams-Eldin H, Pereira IE, Reis AB, Latz A, Heinz D, Aira C, Fresco-Taboada A, Abass E, et al. Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil. Pathogens. 2024; 13(2):109. https://doi.org/10.3390/pathogens13020109
Chicago/Turabian StyleMahdavi, Rouzbeh, Franjo Martinkovic, Hosam Shams-Eldin, Ingrid E. Pereira, Alexandre B. Reis, Andreas Latz, Daniela Heinz, Cristina Aira, Alba Fresco-Taboada, Elfadil Abass, and et al. 2024. "Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil" Pathogens 13, no. 2: 109. https://doi.org/10.3390/pathogens13020109