Screening of Domestic Cats from North-Eastern Hungary for Hepatozoon felis and Cytauxzoon europaeus That Cause Infections in Local Wildcat Populations
Abstract
1. Introduction
2. Materials and Methods
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Target Group | Target Gene | Primer Name | Primer Sequence (5′-3′) | Amplicon Length (bp) | Thermocycling Profile | Reference |
---|---|---|---|---|---|---|
Piroplasms | 18S rRNA | BJ1 | GTC TTG TAA TTG GAA TGA TGG | 500 | 95 °C for 10 min; 40× (95 °C for 30 s; 54 °C for 30 s; 72 °C for 40 s); 72 °C for 5 min | [19] |
BN2 | TAG TTT ATG GTT AGG ACT ACG | |||||
Hepatozoon spp. | 18S rRNA | HepF | ATA CAT GAG CAA AAT CTC AAC | 650 | 95 °C for 5 min; 35× (95 °C for 40 s; 57 °C for 40 s; 72 °C for 60 s); 72 °C for 7 min | [20] |
HepR | CTT ATT ATT CCA TGC TGC AG | |||||
Cytauxzoon spp. | cytb | Cytaux_cytb_Finn | ACC TAC TAA ACC TTA TTC AAG CRT T | 1333 | 95 °C for 5 min; 45× (95 °C for 20 s; 55 °C for 30 s; 68 °C for 1.5 min); 68 °C for 7 min | [21,22] |
Cytaux_cytb_Rinn | AGA CTC TTA GsAT GYA AAC TTC CC |
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Tuska-Szalay, B.; Boldogh, S.A.; Farkas, R.; Rompos, L.; Takács, N.; Beresnyák, V.; Izsó, Á.; Kontschán, J.; Lanszki, J.; Hornok, S. Screening of Domestic Cats from North-Eastern Hungary for Hepatozoon felis and Cytauxzoon europaeus That Cause Infections in Local Wildcat Populations. Pathogens 2023, 12, 656. https://doi.org/10.3390/pathogens12050656
Tuska-Szalay B, Boldogh SA, Farkas R, Rompos L, Takács N, Beresnyák V, Izsó Á, Kontschán J, Lanszki J, Hornok S. Screening of Domestic Cats from North-Eastern Hungary for Hepatozoon felis and Cytauxzoon europaeus That Cause Infections in Local Wildcat Populations. Pathogens. 2023; 12(5):656. https://doi.org/10.3390/pathogens12050656
Chicago/Turabian StyleTuska-Szalay, Barbara, Sándor A. Boldogh, Róbert Farkas, Luca Rompos, Nóra Takács, Viktor Beresnyák, Ádám Izsó, Jenő Kontschán, József Lanszki, and Sándor Hornok. 2023. "Screening of Domestic Cats from North-Eastern Hungary for Hepatozoon felis and Cytauxzoon europaeus That Cause Infections in Local Wildcat Populations" Pathogens 12, no. 5: 656. https://doi.org/10.3390/pathogens12050656
APA StyleTuska-Szalay, B., Boldogh, S. A., Farkas, R., Rompos, L., Takács, N., Beresnyák, V., Izsó, Á., Kontschán, J., Lanszki, J., & Hornok, S. (2023). Screening of Domestic Cats from North-Eastern Hungary for Hepatozoon felis and Cytauxzoon europaeus That Cause Infections in Local Wildcat Populations. Pathogens, 12(5), 656. https://doi.org/10.3390/pathogens12050656