Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Cell Cultivation
2.2. Encystation Assays
2.3. Immunocytochemistry
2.4. Enzyme-Linked Immunosorbent Assay (ELISA)
2.5. Animals and Ethics Requirements
2.6. Establishment of an Animal Model for Acanthamoeba Keratitis
2.7. Detection of Antigen in AK Animal Model Using CM Antibody
2.8. Statistical Analysis
3. Results
3.1. Confirmation of CM Antibody Specificity against Acanthamoeba Trophozoites and Cysts
3.2. Determining the Minimum Detectable Concentration of Acanthamoeba Antigens by CM Antibody
3.3. Construction of the AK Animal Model
3.4. Detection of Acanthamoeba Antigens in the AK Animal Model
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, M.-J.; Jo, H.-J.; Sohn, H.-J.; Shin, H.-J.; Quan, F.-S.; Kong, H.-H.; Moon, E.-K. Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model. Pathogens 2023, 12, 526. https://doi.org/10.3390/pathogens12040526
Kim M-J, Jo H-J, Sohn H-J, Shin H-J, Quan F-S, Kong H-H, Moon E-K. Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model. Pathogens. 2023; 12(4):526. https://doi.org/10.3390/pathogens12040526
Chicago/Turabian StyleKim, Min-Jeong, Hye-Jeong Jo, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, and Eun-Kyung Moon. 2023. "Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model" Pathogens 12, no. 4: 526. https://doi.org/10.3390/pathogens12040526
APA StyleKim, M.-J., Jo, H.-J., Sohn, H.-J., Shin, H.-J., Quan, F.-S., Kong, H.-H., & Moon, E.-K. (2023). Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model. Pathogens, 12(4), 526. https://doi.org/10.3390/pathogens12040526