β-Carboline Alkaloids from Peganum harmala Inhibit Fusarium oxysporum from Codonopsis radix through Damaging the Cell Membrane and Inducing ROS Accumulation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Identification of F. oxysporum
2.2. Chemicals
2.3. Inhibition of Total Alkaloids on Mycelial Growth
2.4. Inhibition of Five βCs on Mycelial Growth and IC50 CALCULATION
2.5. Determination of Minimal Inhibitory Concentration (MIC)
2.6. Scanning Electron Microscopy (SEM)
2.7. Transmission Electron Microscopy (TEM)
2.8. Evaluation of Release of Cell Components
2.9. Measurement of Electrical Conductivity
2.10. ROS Assay
2.11. Annexin V-FITC/PI Double Staining Assay
2.12. Transcriptomic Analysis
2.13. Statistical Analysis
3. Results
3.1. Inhibition of Total Alkaloid Extracts from P. harmala on Mycelial Growth
3.2. Inhibition of Five Target βCs on Mycelial Growth
3.3. MIC
3.4. SEM
3.5. TEM
3.6. Detection of Release of Cell Components and Electrical Conductivity
3.7. Harmane Induced Accumulation of ROS
3.8. Cell Death Analysis
3.9. Effect of Harmane on the Transcriptome of F. oxysporum
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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βCs | Harmaine | Harmaline | Harmalol | Harmane | Harmol |
---|---|---|---|---|---|
IC50 (mg/mL) | 0.143 | 0.331 | 0.798 | 0.050 | 0.161 |
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Zhu, Z.; Zhao, S.; Wang, C. β-Carboline Alkaloids from Peganum harmala Inhibit Fusarium oxysporum from Codonopsis radix through Damaging the Cell Membrane and Inducing ROS Accumulation. Pathogens 2022, 11, 1341. https://doi.org/10.3390/pathogens11111341
Zhu Z, Zhao S, Wang C. β-Carboline Alkaloids from Peganum harmala Inhibit Fusarium oxysporum from Codonopsis radix through Damaging the Cell Membrane and Inducing ROS Accumulation. Pathogens. 2022; 11(11):1341. https://doi.org/10.3390/pathogens11111341
Chicago/Turabian StyleZhu, Zihao, Shujuan Zhao, and Changhong Wang. 2022. "β-Carboline Alkaloids from Peganum harmala Inhibit Fusarium oxysporum from Codonopsis radix through Damaging the Cell Membrane and Inducing ROS Accumulation" Pathogens 11, no. 11: 1341. https://doi.org/10.3390/pathogens11111341