RTA1 Is Involved in Resistance to 7-Aminocholesterol and Secretion of Fungal Proteins in Cryptococcus neoformans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strain Construction
2.2. Resistance to 7-Aminocholesterol
2.3. Capsule Induction
2.4. Capsule Shedding
2.5. Melanin Production
2.6. Urease Production
2.7. Laccase Production
2.8. Phospholipase B Secretion
2.9. Intracellular 7-Aminocholesterol Visualization
2.10. Homology Modeling
2.11. Phylogenetic Analysis
2.12. Promoter Analysis
2.13. Real-Time Quantitative PCR
2.14. Transmission Electron Microscopy (TEM)
2.15. Crystal Structure of 7-Aminocholesterol
2.16. Statistics
3. Results
3.1. Sequence Homology
3.2. Rta1 Phylogeny
3.3. RTA1 Is Involved in Resistance to 7-Aminocholesterol
3.4. Intracellular 7-Aminocholesterol Visualization
3.5. Secretion Phenotype of the H99, rta1Δ, and rta1Δ+RTA1 Strains
3.6. Urease Secretion in Different Secretion Mutants
3.7. qPCR of RTA1 Expression in Different Secretion Mutants
3.8. TEM of the H99, rta1Δ, and rta1Δ+RTA1 Strains
3.9. Potential Model of Rta1 Strucure in the Membrane
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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PDB ID | TC # | Description |
---|---|---|
4OR2 | 9.A.14.7.1 | Human class C G protein-coupled metabotropic glutamate receptor 1 in complex with a negative allosteric modulator |
3RKO | 3.D.1.1.1 | Crystal structure of the membrane domain of respiratory complex I from E. coli at 3.0 angstrom resolution |
3AG3 | 3.D.4.7.1 | Bovine heart cytochrome C oxidase in the nitric oxide-bound fully reduced state at 100 K |
5G28 | 3.E.1.6.14 | The crystal structure of light-driven chloride pump ClR at pH 6.0 |
4HYJ | 3.E.1.6.5 | Crystal structure of Exiguobacterium sibiricum rhodopsin |
3EML | 9.A.14.3.8 | The 2.6 A crystal structure of a human A2A adenosine receptor bound to ZM241385 |
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Smith-Peavler, E.S.; Patel, R.; Onumajuru, A.M.; Bowring, B.G.; Miller, J.L.; Brunel, J.M.; Djordjevic, J.T.; Prabu, M.M.; McClelland, E.E. RTA1 Is Involved in Resistance to 7-Aminocholesterol and Secretion of Fungal Proteins in Cryptococcus neoformans. Pathogens 2022, 11, 1239. https://doi.org/10.3390/pathogens11111239
Smith-Peavler ES, Patel R, Onumajuru AM, Bowring BG, Miller JL, Brunel JM, Djordjevic JT, Prabu MM, McClelland EE. RTA1 Is Involved in Resistance to 7-Aminocholesterol and Secretion of Fungal Proteins in Cryptococcus neoformans. Pathogens. 2022; 11(11):1239. https://doi.org/10.3390/pathogens11111239
Chicago/Turabian StyleSmith-Peavler, Emily S., Ronakkumar Patel, Adejumoke Mary Onumajuru, Bethany G. Bowring, Joyce L. Miller, Jean Michel Brunel, Julianne T. Djordjevic, Moses M. Prabu, and Erin E. McClelland. 2022. "RTA1 Is Involved in Resistance to 7-Aminocholesterol and Secretion of Fungal Proteins in Cryptococcus neoformans" Pathogens 11, no. 11: 1239. https://doi.org/10.3390/pathogens11111239