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Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients

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Department of Surgery-Otolaryngology, Head and Neck Surgery, The University of Adelaide, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville 5011, Australia
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Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar 196, Ethiopia
3
Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne and The Royal Melbourne Hospital, Melbourne 3000, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Bryan Heit
Pathogens 2021, 10(2), 207; https://doi.org/10.3390/pathogens10020207
Received: 14 January 2021 / Revised: 9 February 2021 / Accepted: 11 February 2021 / Published: 14 February 2021
Corynebacterium accolens is the predominant species of the healthy human nasal microbiota, and its relative abundance is decreased in the context of chronic rhinosinusitis (CRS). This study aimed to evaluate the antimicrobial potential of C. accolens isolated from a healthy human nasal cavity against planktonic and biofilm growth of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) clinical isolates (CIs) from CRS patients. Nasal swabs from twenty non-CRS control subjects were screened for the presence of C. accolens using microbiological and molecular techniques. C. accolens CIs and their culture supernatants were tested for their antimicrobial activity against eight S. aureus and eight MRSA 4CIs and S. aureus ATCC25923. The anti-biofilm potential of C. accolens cell-free culture supernatants (CFCSs) on S. aureus biofilms was also assessed. Of the 20 nasal swabs, 10 C. accolens CIs were identified and confirmed with rpoB gene sequencing. All isolates showed variable antimicrobial activity against eight out of 8 S. aureus and seven out of eight MRSA CIs. Culture supernatants from all C. accolens CIs exhibited a significant dose-dependent antibacterial activity (p < 0.05) against five out of five representative S. aureus and MRSA CIs. This inhibition was abolished after proteinase K treatment. C. accolens supernatants induced a significant reduction in metabolic activity and biofilm biomass of S. aureus and MRSA CIs compared to untreated growth control (p < 0.05). C. accolens exhibited antimicrobial activity against S. aureus and MRSA CIs in both planktonic and biofilm forms and holds promise for the development of innovative probiotic therapies to promote sinus health. View Full-Text
Keywords: chronic rhinosinusitis; Corynebacterium accolens; microbiota; sinus health chronic rhinosinusitis; Corynebacterium accolens; microbiota; sinus health
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    Doi: 10.5281/zenodo.4437859
    Link: https://zenodo.org/badge/DOI/10.5281/zenodo.4437859.svg
    Description: Table S1: Description of oligonucleotides used for PCR amplification of rpoB gene in C. accolens isolates. Table S2. Pathogenic S. aureus strains (8MSSA and 8MRSA) isolated from the sinonasal cavity of CRS patients used in this study. Figure S1: Identification of Corynebacterium accolens isolates by PCR amplification of a partial rpoB gene (446-bp fragment).
MDPI and ACS Style

Menberu, M.A.; Liu, S.; Cooksley, C.; Hayes, A.J.; Psaltis, A.J.; Wormald, P.-J.; Vreugde, S. Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients. Pathogens 2021, 10, 207. https://doi.org/10.3390/pathogens10020207

AMA Style

Menberu MA, Liu S, Cooksley C, Hayes AJ, Psaltis AJ, Wormald P-J, Vreugde S. Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients. Pathogens. 2021; 10(2):207. https://doi.org/10.3390/pathogens10020207

Chicago/Turabian Style

Menberu, Martha A., Sha Liu, Clare Cooksley, Andrew J. Hayes, Alkis J. Psaltis, Peter-John Wormald, and Sarah Vreugde. 2021. "Corynebacterium accolens Has Antimicrobial Activity against Staphylococcus aureus and Methicillin-Resistant S. aureus Pathogens Isolated from the Sinonasal Niche of Chronic Rhinosinusitis Patients" Pathogens 10, no. 2: 207. https://doi.org/10.3390/pathogens10020207

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