Differential Incidence of Tongue Base Cancer in Male and Female HPV16-Transgenic Mice: Role of Female Sex Hormone Receptors
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Genotyping
2.3. RNA Extraction and cDNA Synthesis
2.4. qRT-PCR
2.5. Expression of Sex Hormone Receptors: Mouse Experiments
2.6. Histology
2.7. Immunohistochemistry
2.8. Statistical Analysis
3. Results
3.1. Expression of HPV16 E5, E6 and E7 in Male and Female Mice
Experiment 1
3.2. Differential Incidence of Tongue Base Cancer in Male and Female Mice
Experiment 2
3.3. Expression of Female Sex Hormone Receptors in Male and Female Mice
3.4. Expression of MMP2 in Tongue Base Lesions
3.5. Collagen Remodeling in Tongue Base Lesions from Male and Female Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Groups | Normal n (%) | Low-Grade Dysplasia n (%) | High-Grade Dysplasia n (%) | Papilloma n (%) | SCC n (%) |
---|---|---|---|---|---|
Female HPV+ (n = 82) | 0 | 37 (45.1%) | 16 (19.5%) | 10 (12.2%) | 19 (23.2%) |
Female HPV− (n = 10) | 10 (100%) | 0 | 0 | 0 | 0 |
Male HPV+ (n = 67) | 3 (4.5%) | 30 (44.8%) | 19 (28.3%) | 12 (17.9%) | 3 (4.5%) |
Male HPV− (n = 10) | 10 (100%) | 0 | 0 | 0 | 0 |
Sex/Anatomical Site | Low-Grade Dysplasia n | High-Grade Dysplasia n | Papilloma n | SCC n |
---|---|---|---|---|
Females (n = 82) | ||||
Tongue base | 30 | 23 | 10 | 19 |
Rostro-dorsal tongue | 33 | 28 | 2 | 1 |
Ventral tongue | 10 | 2 | 0 | 1 |
Total lesions | 73 | 53 | 12 | 21 |
Average lesions/mouse | 0.89 | 0.64 | 0.15 | 0.26 |
Males (n = 67) | ||||
Tongue base | 30 | 19 | 12 | 3 |
Rostro-dorsal tongue | 30 | 13 | 1 | 0 |
Ventral tongue | 8 | 4 | 0 | 0 |
Total lesions | 68 | 36 | 13 | 3 |
Average lesions/mouse | 1.01 | 0.54 | 0.19 | 0.04 |
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de Oliveira Neto, C.P.; Medeiros-Fonseca, B.; Estêvão, D.; Mestre, V.F.; Costa, N.R.; de Andrade, F.E.; Oliveira, P.A.; Bastos, M.M.S.M.; Medeiros, R.; Assis, D.; et al. Differential Incidence of Tongue Base Cancer in Male and Female HPV16-Transgenic Mice: Role of Female Sex Hormone Receptors. Pathogens 2021, 10, 1224. https://doi.org/10.3390/pathogens10101224
de Oliveira Neto CP, Medeiros-Fonseca B, Estêvão D, Mestre VF, Costa NR, de Andrade FE, Oliveira PA, Bastos MMSM, Medeiros R, Assis D, et al. Differential Incidence of Tongue Base Cancer in Male and Female HPV16-Transgenic Mice: Role of Female Sex Hormone Receptors. Pathogens. 2021; 10(10):1224. https://doi.org/10.3390/pathogens10101224
Chicago/Turabian Stylede Oliveira Neto, Clariano Pires, Beatriz Medeiros-Fonseca, Diogo Estêvão, Verónica F. Mestre, Natália R. Costa, Fábio Evangelista de Andrade, Paula A. Oliveira, Margarida M. S. M. Bastos, Rui Medeiros, Diogo Assis, and et al. 2021. "Differential Incidence of Tongue Base Cancer in Male and Female HPV16-Transgenic Mice: Role of Female Sex Hormone Receptors" Pathogens 10, no. 10: 1224. https://doi.org/10.3390/pathogens10101224