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Open AccessArticle

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
National Institute of Materials Physics, P.O. Box MG-7, 76900 Magurele-Bucharest, Romania
Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons-Hainaut, 24, Avenue du Champ de Mars, B-7000 Mons, Belgium
Department of Agricultural Development, Democritus University of Thrace, 67100 Xanthi, Greece
Authors to whom correspondence should be addressed.
Academic Editor: Alexandru Mihai Grumezescu
Metals 2017, 7(2), 63;
Received: 19 November 2016 / Revised: 6 February 2017 / Accepted: 15 February 2017 / Published: 21 February 2017
(This article belongs to the Special Issue Metallic and Metal Oxide Nanoparticles: Novel Approaches)
We report the synthesis of dextran-coated iron oxide magnetic nanoparticles (DIO-NPs) with spherical shape and uniform size distribution as well as their accumulation and toxic effects on Jurkat cells up to 72 h. The characterization of dextran-coated maghemite nanoparticles was done by X-ray diffraction and dynamic light scattering analyses, transmission electron microscopy imaging, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, magnetic hysteresis, and relaxometry measurements. The quantification of DIO-NPs intracellular uptake showed a progressive accumulation of iron as a function of time and dose accompanied by additional lysosome formation and an increasing darkening exhibited by a magnetic resonance imaging (MRI) scanner. The cytotoxicity assays revealed a decrease of cell viability and a loss of membrane integrity in a time- and dose-dependent manner. Exposure to DIO-NPs determined an increase in reactive oxygen species level up to 72 h. In the first two days of exposure, the level of reduced glutathione decreased and the amount of malondyaldehyde increased, but at the end of the experiment, their concentrations returned to control values. These nanoparticles could be used as contrast agents for MRI but several parameters concerning their interaction with the cells should be taken into consideration for a safe utilization. View Full-Text
Keywords: dextran; maghemite; T lymphocyte cells; iron bioaccumulation; cytotoxicity dextran; maghemite; T lymphocyte cells; iron bioaccumulation; cytotoxicity
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Balas, M.; Ciobanu, C.S.; Burtea, C.; Stan, M.S.; Bezirtzoglou, E.; Predoi, D.; Dinischiotu, A. Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles. Metals 2017, 7, 63.

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