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Open AccessArticle

Effects of Surface Structure and Chemical Composition of Binary Ti Alloys on Cell Differentiation

Department of Dental Materials and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju 61186, Korea
Author to whom correspondence should be addressed.
Academic Editor: Rita Khanna
Metals 2016, 6(7), 150;
Received: 1 March 2016 / Revised: 21 May 2016 / Accepted: 30 June 2016 / Published: 4 July 2016
(This article belongs to the Special Issue Refractory Metals and Alloys)
Binary Ti alloys containing Fe, Mo, V and Zr were micro-arc oxidized and hydrothermally treated to obtain micro- and nano-porous layers. This study aimed to investigate cell differentiation on micro and micro/nanoporous oxide layers of Ti alloys. The properties of the porous layer formed on Ti alloys were characterized by X-ray diffraction pattern, microstructural and elemental analyses and inductively coupled plasma mass spectrometry (ICP-MS) method. The MTT assay, total protein production and alkaline phosphatase (ALPase) activity were evaluated using human osteoblast-like cells (MG-63). Microporous structures of micro-arc oxidized Ti alloys were changed to micro/nanoporous surfaces after hydrothermal treatment. Micro/nanoporous surfaces consisted of acicular TiO2 nanoparticles and micron-sized hydroxyapatite particles. From ICP and MTT tests, the Mo and V ions released from porous oxide layers were positive for cell viability, while the released Fe ions were negative for cell viability. Although the micro/nanoporous surfaces led to a lower total protein content than the polished and microporous Ti surfaces after cell incubation for 7 days, they caused higher ALPase activities after 7 days and 14 days of incubation except for V-containing microporous surfaces. The micro/nanoporous surfaces of Ti alloys were more efficient in inducing MG-63 cell differentiation. View Full-Text
Keywords: Ti alloys; micro-arc oxidation; hydrothermal treatment; cell differentiation Ti alloys; micro-arc oxidation; hydrothermal treatment; cell differentiation
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MDPI and ACS Style

Han, O.-S.; Hwang, M.-J.; Song, Y.-H.; Song, H.-J.; Park, Y.-J. Effects of Surface Structure and Chemical Composition of Binary Ti Alloys on Cell Differentiation. Metals 2016, 6, 150.

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