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Article

The Effects of Chemical Etching and Ultra-Fine Grain Structure of Titanium on MG-63 Cells Response

1
Saint Petersburg State University, Universitetskaya Nab, 7/9, 199034 Saint Petersburg, Russia
2
Peter the Great Saint Petersburg Polytechnic University, Polytechnicheskaya 29, 195221 Saint Petersburg, Russia
3
Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave. 4, 194064 Saint Petersburg, Russia
4
Radiation Immuno-Oncology Group, Center for Translational Cancer Research Technische Universität München (TranslaTUM), Klinikum Rechts der Isar, Einstein Str. 25, 81675 Munich, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Elena Gordo
Metals 2021, 11(3), 510; https://doi.org/10.3390/met11030510
Received: 17 February 2021 / Revised: 12 March 2021 / Accepted: 16 March 2021 / Published: 19 March 2021
(This article belongs to the Special Issue Surface Modification of Metallic Biomaterials)
In this work, we study the influence of the surface properties of ultrafine grained (UFG) and coarse grained (CG) titanium on the morphology, viability, proliferation and differentiation of osteoblast-like MG-63 cells. Wet chemical etching in H2SO4/H2O2 and NH4OH/H2O2 solutions was used for producing surfaces with varying morphology, topography, composition and wettability. The topography and morphology have been studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The composition was determined by time of flight mass-spectrometry (TOF-SIMS) and X-ray photoelectron spectroscopy (XPS). The results showed that it is possible to obtain samples with different compositions, hydrophilicity, topography and nanoscale or/and microscale structures by changing the etching time and the type of etching solution. It was found that developed topography and morphology can improve spreading and proliferation rate of MG-63 cells. A significant advantage of the samples of the UFG series in comparison with CG in adhesion, proliferation at later stages of cultivation (7 days), higher alkaline phosphatase (ALP) activity and faster achievement of its maximum values was found. However, there is no clear benefit of the UFG series on osteopontin (OPN) expression. All studied samples showed no cytotoxicity towards MG-63 cells and promoted their osteogenic differentiation. View Full-Text
Keywords: titanium; ultrafine grained materials; chemical etching; surface science; osteoblast response titanium; ultrafine grained materials; chemical etching; surface science; osteoblast response
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MDPI and ACS Style

Nazarov, D.; Zemtsova, E.; Smirnov, V.; Mitrofanov, I.; Maximov, M.; Yudintceva, N.; Shevtsov, M. The Effects of Chemical Etching and Ultra-Fine Grain Structure of Titanium on MG-63 Cells Response. Metals 2021, 11, 510. https://doi.org/10.3390/met11030510

AMA Style

Nazarov D, Zemtsova E, Smirnov V, Mitrofanov I, Maximov M, Yudintceva N, Shevtsov M. The Effects of Chemical Etching and Ultra-Fine Grain Structure of Titanium on MG-63 Cells Response. Metals. 2021; 11(3):510. https://doi.org/10.3390/met11030510

Chicago/Turabian Style

Nazarov, Denis, Elena Zemtsova, Vladimir Smirnov, Ilya Mitrofanov, Maxim Maximov, Natalia Yudintceva, and Maxim Shevtsov. 2021. "The Effects of Chemical Etching and Ultra-Fine Grain Structure of Titanium on MG-63 Cells Response" Metals 11, no. 3: 510. https://doi.org/10.3390/met11030510

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