A C-Type Lectin, RfCTL27, Activates the Immune Defense in the Red Palm Weevil Rhynchophorus ferrugineus (A.G. Olivier, 1791) (Coleoptera: Curculionidae: Dryophthorinae) by the Recognition of Gram-Negative Bacteria
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Red Palm Weevil Rearing and Breeding
2.2. Sequence Characterization of RfCTL27
2.3. Analysis of RfCTL27 Expression in Different Tissues of the RPW Larvae
2.4. Expression of RfCTL27 in the Gut and Fat Body of RPW Larvae Challenge with Pathogenic Microbes
2.5. The Impact of RfCTL27 Knockdown on RPW Larvae Immune Response and the Count of Gut eGFP-Labeled E. coli Colonies
2.6. Data Analysis and Processing
3. Results
3.1. Sequence Characteristics of RfCTL27
3.2. Expression Profiles of RfCTL27 in the Tissues of RPW Larvae
3.3. Expression Patterns of RfCTL27 upon Pathogen Challenge in RPW Larvae
3.4. Effect of RfCTL27 Silencing on the Immunocompetence of RPW Larvae and Expression of Antimicrobial Peptide Genes in the Gut and Fat Body
3.5. The Effect of RfCTL27 Knockdown on the Ability of RPW Larvae to Clear to the Invaded E. coli in Hemolymph
4. Discussion
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Reagent | Dosage |
---|---|
SYBR mix | 10 μL |
Forward primer | 0.4 μL |
Reverse primer | 0.4 μL |
cDNA | 1 μL |
ddH2O | 8.2 μL |
Total | 20 μL |
Primers | Sequences (5′-3′) |
---|---|
RT-qPCR | |
Rfβ-Actin F | CCAAGGGAGCCAAGCAATT |
Rfβ-Actin R | CGCTGATGCCCCTATGTATGT |
RfCTL27 F | ATCAACGGATGGTTCTGGTC |
RfCTL27 R | ACGAAGGGTTTCAGATGGT |
RfAttacin F | TGGTTCTGGTGCCCAAGTGA |
RfAttacin R | GCCATAACGATTCTTGTTGGAGTA |
RfCecropin F | CAGAAGCTGGTTGGTTGAAGA |
RfCecropin R | GCAACACCGACATAACCCTGA |
RfColeopericin F | TCGTGGTTTCTACCATGTTCACT |
RfColeopericin R | TCAGCTAAAACCTGATCTTGGA |
RfDefensin F | TTCGCCAAACTTATCCTCGTG |
RfDefensin R | GGGTGCTTCGTTATCAACTTCC |
RNAi | |
RfdsCTL27 F | taatacgactcactatagggACCTGGAAGTCGACTGGTTG |
RfdsCTL27 R | taatacgactcactatagggAGTTCCTCGCTATCTTCGCA |
RfdseGFP F | taatacgactcactatagggCAGTGCTTCAGCCGCTAC |
RfdseGFP R | taatacgactcactatagggGTTCACCTGCCGTTCTTGA |
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Gong, Y.; Xia, Y.; Su, Z.; Wang, X.; Kou, Y.; Ma, B.; Hou, Y.; Shi, Z. A C-Type Lectin, RfCTL27, Activates the Immune Defense in the Red Palm Weevil Rhynchophorus ferrugineus (A.G. Olivier, 1791) (Coleoptera: Curculionidae: Dryophthorinae) by the Recognition of Gram-Negative Bacteria. Insects 2024, 15, 212. https://doi.org/10.3390/insects15030212
Gong Y, Xia Y, Su Z, Wang X, Kou Y, Ma B, Hou Y, Shi Z. A C-Type Lectin, RfCTL27, Activates the Immune Defense in the Red Palm Weevil Rhynchophorus ferrugineus (A.G. Olivier, 1791) (Coleoptera: Curculionidae: Dryophthorinae) by the Recognition of Gram-Negative Bacteria. Insects. 2024; 15(3):212. https://doi.org/10.3390/insects15030212
Chicago/Turabian StyleGong, Yanru, Yongjian Xia, Zhiping Su, Xinghong Wang, Yishuo Kou, Bing Ma, Youming Hou, and Zhanghong Shi. 2024. "A C-Type Lectin, RfCTL27, Activates the Immune Defense in the Red Palm Weevil Rhynchophorus ferrugineus (A.G. Olivier, 1791) (Coleoptera: Curculionidae: Dryophthorinae) by the Recognition of Gram-Negative Bacteria" Insects 15, no. 3: 212. https://doi.org/10.3390/insects15030212