An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera
Abstract
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Source of Insect Strains
2.2. Details of the Rearing Protocol
2.2.1. Rearing Conditions
2.2.2. Preparation of Sprouted Corn
2.2.3. Single-Pair and Colony-Level Egg Laying
2.2.4. Egg Collection and Incubation
2.2.5. Preparing Primary Rearing Containers
2.2.6. Preparing Secondary and Tertiary Rearing Containers
2.2.7. Preparing Pupal Rearing Containers
2.2.8. Adult Collection for Colony- or Single-Pair Mating
2.3. Development Time, Eclosion Efficiency after Handling, and Quality Control
2.4. Fluorescent Light Screening
2.5. Single Pupae Handling and Survival Rate
2.6. Testing Single-Pair Mating Scheme
3. Results
3.1. Developmental Rate
3.2. Survival Rate
3.3. Quality Control System
3.4. Impact of Fluorescent Light Screening
3.5. Handling and Sexing Pupae
3.6. Single-Pair Crosses
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Stage | # of Insects | # of Adults | Survival Rate |
---|---|---|---|
Larvae | 3260 | 2675 | 82.06% |
Pre/pupae 1 | 2184 | 1223 | 56.00% |
Mix 2 | 2146 | 1586 | 73.90% |
Total | 7590 | 5484 | 72.25% |
Life Stage | # of Insects | # of Adults | Eclosion Rate |
---|---|---|---|
Larvae | 480 | 438 | 91.25% |
Pre-pupae | 2630 | 1798 | 68.37% |
Pupae | 4766 | 3950 | 82.88% |
Pre/pupae | 818 | 652 | 79.71% |
This Work | 1973 * | 1972 + | ||||
---|---|---|---|---|---|---|
Range | Mean | Range | Mean | Range | Mean | |
Female longevity (days) | 31–132 | 78.4 ± 32.6 | 6–163 | 67.7 ± 30.6 | 19–126 | 94.8 ± 12.5 |
No. eggs oviposited/female | 83–1070 | 414 ± 290.3 | 237–912 | 593.l ± 231.3 | 85–1913 | 1023 ± 240 |
No. clutches/female | 3–17 | 8.1 ± 5 | 3–20 | 11.3 ± 5.4 | ||
Avg no. eggs/clutch/female | 1–128 | 54.42 ± 25.7 | 39–79 | 56.9 ± 14.2 | ||
Avg days between ovarian cycles | 1–11 | 4.63 ± 2.3 | 4.2–6.0 | 4.9 ± 0.4 | ||
Preoviposition period (days) | 13–40 | 19.3 ± 8 | 11–19 | 15.3 ± 2.0 |
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Chu, F.-C.; Wu, P.-S.; Pinzi, S.; Grubbs, N.; Cohen, A.C.; Lorenzen, M.D. An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera. Insects 2023, 14, 683. https://doi.org/10.3390/insects14080683
Chu F-C, Wu P-S, Pinzi S, Grubbs N, Cohen AC, Lorenzen MD. An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera. Insects. 2023; 14(8):683. https://doi.org/10.3390/insects14080683
Chicago/Turabian StyleChu, Fu-Chyun, Pei-Shan Wu, Sofia Pinzi, Nathaniel Grubbs, Allen Carson Cohen, and Marcé D. Lorenzen. 2023. "An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera" Insects 14, no. 8: 683. https://doi.org/10.3390/insects14080683
APA StyleChu, F.-C., Wu, P.-S., Pinzi, S., Grubbs, N., Cohen, A. C., & Lorenzen, M. D. (2023). An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera. Insects, 14(8), 683. https://doi.org/10.3390/insects14080683