Next Article in Journal
Immigrant Tortricidae: Holarctic versus Introduced Species in North America
Previous Article in Journal
Temporal Dynamics of ‘Ca. Phytoplasma mali’ Load in the Insect Vector Cacopsylla melanoneura
Article

Bacteria Belonging to Pseudomonas typographi sp. nov. from the Bark Beetle Ips typographus Have Genomic Potential to Aid in the Host Ecology

1
Microbiology and Genetics Department, University of Salamanca, 37007 Salamanca, Spain
2
Spanish-Portuguese Institute for Agricultural Research (CIALE), 37185 Salamanca, Spain
3
Department of Botany, Faculty of Science, Charles University, Benátská 2, 128 01 Prague, Czech Republic
4
Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic
5
Associated Research Unit of Plant-Microorganism Interaction, University of Salamanca-IRNASA-CSIC, 37008 Salamanca, Spain
*
Author to whom correspondence should be addressed.
Insects 2020, 11(9), 593; https://doi.org/10.3390/insects11090593
Received: 4 July 2020 / Revised: 23 August 2020 / Accepted: 1 September 2020 / Published: 3 September 2020
European Bark Beetle (Ips typographus) is a pest that affects dead and weakened spruce trees. Under certain environmental conditions, it has massive outbreaks, resulting in attacks of healthy trees, becoming a forest pest. It has been proposed that the bark beetle’s microbiome plays a key role in the insect’s ecology, providing nutrients, inhibiting pathogens, and degrading tree defense compounds, among other probable traits. During a study of bacterial associates from I. typographus, we isolated three strains identified as Pseudomonas from different beetle life stages. In this work, we aimed to reveal the taxonomic status of these bacterial strains and to sequence and annotate their genomes to mine possible traits related to a role within the bark beetle holobiont. Our study indicates that these bacteria constitute a new species for which the name of Pseudomonas typographi sp. nov. is proposed. Moreover, their genome analysis suggests different metabolic pathways possibly related to the beetle’s ecology. Finally, in vitro tests conclude the capability of these bacteria to inhibit beetle’s fungal pathogens. Altogether, these results suggest that P. typographi aids I. typographi nutrition and resistance to fungal pathogens. These findings might be of interest in the development of integrated methods for pest control.
European Bark Beetle Ips typographus is a secondary pest that affects dead and weakened spruce trees (Picea genus). Under certain environmental conditions, it has massive outbreaks, resulting in the attacks of healthy trees, becoming a forest pest. It has been proposed that the bark beetle’s microbiome plays a key role in the insect’s ecology, providing nutrients, inhibiting pathogens, and degrading tree defense compounds, among other probable traits yet to be discovered. During a study of bacterial associates from I. typographus, we isolated three strains identified as Pseudomonas from different beetle life stages. A polyphasic taxonomical approach showed that they belong to a new species for which the name Pseudomonas typographi sp nov. is proposed. Genome sequences show their potential to hydrolyze wood compounds and synthesize several vitamins; screening for enzymes production was verified using PNP substrates. Assays in Petri dishes confirmed cellulose and xylan hydrolysis. Moreover, the genomes harbor genes encoding chitinases and gene clusters involved in the synthesis of secondary metabolites with antimicrobial potential. In vitro tests confirmed the capability of the three P. typographi strains to inhibit several Ips beetles’ pathogenic fungi. Altogether, these results suggest that P. typographi aids I. typographi nutrition and resistance to fungal pathogens. View Full-Text
Keywords: Ips typographus; bark beetle; bacteriome; Pseudomonas; beneficial Ips typographus; bark beetle; bacteriome; Pseudomonas; beneficial
Show Figures

Figure 1

MDPI and ACS Style

Peral-Aranega, E.; Saati-Santamaría, Z.; Kolařik, M.; Rivas, R.; García-Fraile, P. Bacteria Belonging to Pseudomonas typographi sp. nov. from the Bark Beetle Ips typographus Have Genomic Potential to Aid in the Host Ecology. Insects 2020, 11, 593. https://doi.org/10.3390/insects11090593

AMA Style

Peral-Aranega E, Saati-Santamaría Z, Kolařik M, Rivas R, García-Fraile P. Bacteria Belonging to Pseudomonas typographi sp. nov. from the Bark Beetle Ips typographus Have Genomic Potential to Aid in the Host Ecology. Insects. 2020; 11(9):593. https://doi.org/10.3390/insects11090593

Chicago/Turabian Style

Peral-Aranega, Ezequiel, Zaki Saati-Santamaría, Miroslav Kolařik, Raúl Rivas, and Paula García-Fraile. 2020. "Bacteria Belonging to Pseudomonas typographi sp. nov. from the Bark Beetle Ips typographus Have Genomic Potential to Aid in the Host Ecology" Insects 11, no. 9: 593. https://doi.org/10.3390/insects11090593

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop