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Article

XadA2 Adhesin Decreases Biofilm Formation and Transmission of Xylella fastidiosa subsp. pauca

1
Departamento de Entomologia e Acarologia, Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), Universidade de São Paulo (USP), Piracicaba, SP 13418-900, Brazil
2
Centro de Citricultura “Sylvio Moreira”, Instituto Agronômico, Cordeirópolis, SP 13490-970, Brazil
3
Instituto Federal do Pará, Castanhal, Pará 68740-970, Brazil
4
Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720-3114, USA
*
Author to whom correspondence should be addressed.
Insects 2020, 11(8), 473; https://doi.org/10.3390/insects11080473
Received: 10 June 2020 / Revised: 21 July 2020 / Accepted: 21 July 2020 / Published: 26 July 2020
(This article belongs to the Special Issue Insect Vectors of Plant Pathogens)
Xylella fastidiosa is a vector-borne bacterium that causes diseases in many plants of economic interest. The bacterium–vector initial interactions involve bacterial membrane-bound adhesins that mediate cell attachment to the foregut of insect vectors. We investigated the role of the afimbrial adhesin XadA2 in the binding and biofilm formation of X. fastidiosa subsp. pauca to vector surfaces in vitro, as well as its potential to disrupt pathogen transmission. We showed that XadA2 has binding affinity for polysaccharides on sharpshooter hindwings, used as a proxy for the interactions between X. fastidiosa and vectors. When in a medium without carbon sources, the bacterium used wing components, likely chitin, as a source of nutrients and formed a biofilm on the wing surface. There was a significant reduction in X. fastidiosa biofilm formation and cell aggregation on vector wings in competition assays with XadA2 or its specific antibody (anti-XadA2). Finally, pathogen acquisition and transmission to plant were significantly reduced when the vectors acquired X. fastidiosa from an artificial diet supplemented with anti-XadA2. These results show that XadA2 is important in mediating bacterial colonization in the insect and that it could be used as a target for blocking X. fastidiosa transmission. View Full-Text
Keywords: sharpshooter; vector-borne; attachment; bacterium; blocking transmission sharpshooter; vector-borne; attachment; bacterium; blocking transmission
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MDPI and ACS Style

Bossi Esteves, M.; Lopes Nalin, J.; Kudlawiec, K.; Caserta Salviatto, R.; de Melo Sales, T.; Sicard, A.; Piacentini Paes de Almeida, R.; Alves de Souza, A.; Roberto Spotti Lopes, J. XadA2 Adhesin Decreases Biofilm Formation and Transmission of Xylella fastidiosa subsp. pauca. Insects 2020, 11, 473. https://doi.org/10.3390/insects11080473

AMA Style

Bossi Esteves M, Lopes Nalin J, Kudlawiec K, Caserta Salviatto R, de Melo Sales T, Sicard A, Piacentini Paes de Almeida R, Alves de Souza A, Roberto Spotti Lopes J. XadA2 Adhesin Decreases Biofilm Formation and Transmission of Xylella fastidiosa subsp. pauca. Insects. 2020; 11(8):473. https://doi.org/10.3390/insects11080473

Chicago/Turabian Style

Bossi Esteves, Mariana, Julia Lopes Nalin, Karla Kudlawiec, Raquel Caserta Salviatto, Tiago de Melo Sales, Anne Sicard, Rodrigo Piacentini Paes de Almeida, Alessandra Alves de Souza, and João Roberto Spotti Lopes. 2020. "XadA2 Adhesin Decreases Biofilm Formation and Transmission of Xylella fastidiosa subsp. pauca" Insects 11, no. 8: 473. https://doi.org/10.3390/insects11080473

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