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Article

Identification and Expression of Inward-Rectifying Potassium Channel Subunits in Plutella xylostella

1
Long Ping Branch, Graduate School of Hunan University, Changsha 410125, China
2
Institute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, China
*
Authors to whom correspondence should be addressed.
Insects 2020, 11(8), 461; https://doi.org/10.3390/insects11080461
Received: 10 July 2020 / Revised: 20 July 2020 / Accepted: 21 July 2020 / Published: 22 July 2020
(This article belongs to the Special Issue Insect Molecular Biology)
In insects, inward-rectifying potassium (Kir) channels regulate vital physiological functions, such as feeding behavior, silk secretion, renal excretion, and immune function. Therefore, they offer promising potential as targets for insecticides. Three types of Kir subunits have been identified in Diptera and Hemiptera, but the Kir subunits of Lepidoptera still remain unclear. This study identified five Kir subunit genes (pxkir1, pxkir2, pxkir3A, pxkir3B, and pxkir4) in the transcriptome of Plutella xylostella. Phylogenetic analysis identified pxkir1, pxkir2, pxkir3A, and pxkir3B as orthologous genes of kir13 in other insects. Interestingly, pxkir4 may be encoding a new class of Kir subunit in Lepidoptera that has not been reported to date. To identify further Kir channel subunits of P. xylostella, the gene expression profiles of five pxkir genes were studied by quantitative real-time PCR. These pxkir genes are expressed throughout the development of P. xylostella. pxkir1 and pxkir2 were highly expressed in thoraxes and legs, while pxkir3 (3A and 3B) and pxkir4 had high expression levels in the midgut and Malpighian tubules. This study identified the composition and distribution of Kir subunits in P. xylostella for the first time, and provides useful information for the further study of Kir channel subunits in Lepidoptera. View Full-Text
Keywords: Plutella xylostella; Kir channel; phylogenetic analysis; real-time PCR Plutella xylostella; Kir channel; phylogenetic analysis; real-time PCR
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MDPI and ACS Style

Lai, X.; Xu, J.; Ma, H.; Liu, Z.; Zheng, W.; Liu, J.; Zhu, H.; Zhou, Y.; Zhou, X. Identification and Expression of Inward-Rectifying Potassium Channel Subunits in Plutella xylostella. Insects 2020, 11, 461. https://doi.org/10.3390/insects11080461

AMA Style

Lai X, Xu J, Ma H, Liu Z, Zheng W, Liu J, Zhu H, Zhou Y, Zhou X. Identification and Expression of Inward-Rectifying Potassium Channel Subunits in Plutella xylostella. Insects. 2020; 11(8):461. https://doi.org/10.3390/insects11080461

Chicago/Turabian Style

Lai, Xiaoyi, Jie Xu, Haihao Ma, Zheming Liu, Wei Zheng, Jia Liu, Hang Zhu, Yong Zhou, and Xiaomao Zhou. 2020. "Identification and Expression of Inward-Rectifying Potassium Channel Subunits in Plutella xylostella" Insects 11, no. 8: 461. https://doi.org/10.3390/insects11080461

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