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Open AccessArticle

Transcriptomic Analysis of Mating Responses in Bemisia tabaci MED Females

1
Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China
2
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3
Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA
*
Authors to whom correspondence should be addressed.
Authors contributed equally to this work.
Insects 2020, 11(5), 308; https://doi.org/10.3390/insects11050308
Received: 12 March 2020 / Revised: 7 May 2020 / Accepted: 11 May 2020 / Published: 14 May 2020
Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up- and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.
Keywords: Bemisia tabaci MED; transcriptome; mating responses; DEG; female Bemisia tabaci MED; transcriptome; mating responses; DEG; female
MDPI and ACS Style

Huo, Z.; Liu, Y.; Yang, J.; Xie, W.; Wang, S.; Wu, Q.; Zhou, X.; Pang, B.; Zhang, Y. Transcriptomic Analysis of Mating Responses in Bemisia tabaci MED Females. Insects 2020, 11, 308.

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