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Open AccessArticle

De novo Transcriptomic Resources in the Brain of Vespa velutina for Invasion Control

1
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
2
College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China
3
College of Animal Science, Zhejiang University, Hangzhou 310058, China
*
Author to whom correspondence should be addressed.
Insects 2020, 11(2), 101; https://doi.org/10.3390/insects11020101
Received: 3 December 2019 / Revised: 14 January 2020 / Accepted: 1 February 2020 / Published: 3 February 2020
The invasion of Vespa velutina presents a great threat to the agriculture economy, the ecological environment, and human health. An effective strategy for this hornet control is urgently required, but the limited genome information of Vespa velutina restricts the application of molecular-genomic tools for targeted hornet management. Therefore, we conducted large-scale transcriptome profiling of the hornet brain to obtain functional target genes and molecular markers. Using an Illumina HiSeq platform, more than 41 million clean reads were obtained and de novo assembled into 182,087 meaningful unigenes. A total of 56,400 unigenes were annotated against publicly available protein sequence databases and a set of reliable Simple Sequence Repeats (SSRs) and Single Nucleotide Polymorphisms (SNP) markers were developed. The homologous genes encoding crucial behavior regulation factors, odorant binding proteins (OBPs), and vitellogenin, were also identified from highly expressed transcripts. This study provides abundant molecular targets and markers for invasive hornet control and further promotes the genetic and molecular study of Vespa velutina.
Keywords: Vespa velutina; hornet; invasion control; de novo transcriptome Vespa velutina; hornet; invasion control; de novo transcriptome
MDPI and ACS Style

Wang, M.; Li, H.; Zheng, H.; Zhao, L.; Xue, X.; Wu, L. De novo Transcriptomic Resources in the Brain of Vespa velutina for Invasion Control. Insects 2020, 11, 101.

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