RNA Interference-Based Silencing of the Chitin Synthase 1 Gene for Reproductive and Developmental Disruptions in Panonychus citri
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Rearing of Panonychus citri
2.2. Expression Profiles of the Target Gene
2.3. Preparation of Target Gene Double-Stranded RNA (dsRNA)
2.4. Sample Preparation for RNAi Bioassays
2.5. Impacst of the Target Gene dsRNA on the Expression Patterns of Chitin Synthase 1 Gene Regulation in Different Stages of Panonychus citri
2.6. Time–Mortality Response of the Panonychus citri to Target Gene dsRNAs
2.7. Effects of Target Gene dsRNA on the Reproduction Potential of the Panonychus citri
2.8. Impact of the Target Gene dsRNA on the Chitin Contents of Panonychus citri
2.9. Histological Analysis to Observe Alterations
3. Results
3.1. Expression Profile of the PcCHS1Gene in Different Developmental Stages of the Panonychus citri
3.2. RNAi-Based Silencing of PcCHS1 in Different Developmental Stages of the Panonychus citri
3.3. Time–Mortality Response in Different Developmental Stages of the Panonychus citri
3.4. Impact of Target Gene Silencing on the Reproduction Potential of Panonychus citri
3.5. Disruptions in Chitin Contents
3.6. Cuticular Disruption and Cellular Abnormalities
3.7. Chitin Reduction in the Cuticles of Panonychus citri
3.8. Transmission Electron Microscopic Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Ali, M.W.; Khan, M.M.; Song, F.; Wu, L.; He, L.; Wang, Z.; Zhang, Z.-y.; Zhang, H.; Jiang, Y. RNA Interference-Based Silencing of the Chitin Synthase 1 Gene for Reproductive and Developmental Disruptions in Panonychus citri. Insects 2020, 11, 786. https://doi.org/10.3390/insects11110786
Ali MW, Khan MM, Song F, Wu L, He L, Wang Z, Zhang Z-y, Zhang H, Jiang Y. RNA Interference-Based Silencing of the Chitin Synthase 1 Gene for Reproductive and Developmental Disruptions in Panonychus citri. Insects. 2020; 11(11):786. https://doi.org/10.3390/insects11110786
Chicago/Turabian StyleAli, Muhammad Waqar, Muhammad Musa Khan, Fang Song, Liming Wu, Ligang He, Zhijing Wang, Zhen-yu Zhang, Hongyu Zhang, and Yingchun Jiang. 2020. "RNA Interference-Based Silencing of the Chitin Synthase 1 Gene for Reproductive and Developmental Disruptions in Panonychus citri" Insects 11, no. 11: 786. https://doi.org/10.3390/insects11110786