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Article

BmFoxO Gene Regulation of the Cell Cycle Induced by 20-Hydroxyecdysone in BmN-SWU1 Cells

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China
2
Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400715, China
*
Authors to whom correspondence should be addressed.
Insects 2020, 11(10), 700; https://doi.org/10.3390/insects11100700
Received: 10 September 2020 / Revised: 11 October 2020 / Accepted: 12 October 2020 / Published: 14 October 2020
Ecdysteroid titer determines the state of the cell cycle in silkworm (Bombyx mori) metamorphosis. However, the mechanism of this process is unclear. In this study, we reported that 20-Hydroxyecdysone (20E) can promote BmFoxO (Bombyx mori Forkhead box protein O) gene expression and induce BmFoxO nuclear translocation in BmN-SWU1 cells. Overexpression of the BmFoxO gene affects cell cycle progression, which results in cell cycle arrest in the G0/G1 phase as well as inhibition of DNA replication. Further investigations showed that the effect of 20E was attenuated after BmFoxO gene knockdown. The findings of this study confirmed that BmFoxO is a key mediator in the cell cycle regulation pathway induced by 20E. This suggests a novel pathway for ecdysteroid-induced cell cycle regulation in the process of silkworm metamorphosis, and it is likely to be conserved between Lepidoptera insects.
Ecdysteroid titer determines the state of the cell cycle in silkworm (Bombyxmori) metamorphosis. However, the mechanism of this process is unclear. In this study, we demonstrated that the BmFoxO gene participates in the regulation of the cell cycle induced by 20-Hydroxyecdysone (20E) in BmN-SWU1 cells. The 20E blocks the cell cycle in the G2/M phase through the ecdysone receptor (EcR) and inhibits DNA replication. The 20E can promote BmFoxO gene expression. Immunofluorescence and Western blot results indicated that 20E can induce BmFoxO nuclear translocation in BmN-SWU1 cells. Overexpression of the BmFoxO gene affects cell cycle progression, which results in cell cycle arrest in the G0/G1 phase as well as inhibition of DNA replication. Knockdown of the BmFoxO gene led to cell accumulation at the G2/M phase. The effect of 20E was attenuated after BmFoxO gene knockdown. These findings increase our understanding of the function of 20E in the regulation of the cell cycle in B. mori. View Full-Text
Keywords: 20-Hydroxyecdysone; BmFoxO; Bombyx mori; cell cycle; developmental switching; EcR 20-Hydroxyecdysone; BmFoxO; Bombyx mori; cell cycle; developmental switching; EcR
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MDPI and ACS Style

Zhang, Q.; Yang, J.; Chen, P.; Liu, T.; Xiao, Q.; Zhou, X.; Wang, L.; Long, Y.; Dong, Z.; Pan, M.; Lu, C. BmFoxO Gene Regulation of the Cell Cycle Induced by 20-Hydroxyecdysone in BmN-SWU1 Cells. Insects 2020, 11, 700. https://doi.org/10.3390/insects11100700

AMA Style

Zhang Q, Yang J, Chen P, Liu T, Xiao Q, Zhou X, Wang L, Long Y, Dong Z, Pan M, Lu C. BmFoxO Gene Regulation of the Cell Cycle Induced by 20-Hydroxyecdysone in BmN-SWU1 Cells. Insects. 2020; 11(10):700. https://doi.org/10.3390/insects11100700

Chicago/Turabian Style

Zhang, Qian, Jigui Yang, Peng Chen, Taihang Liu, Qin Xiao, Xiaolin Zhou, Ling Wang, Yanbi Long, Zhanqi Dong, Minhui Pan, and Cheng Lu. 2020. "BmFoxO Gene Regulation of the Cell Cycle Induced by 20-Hydroxyecdysone in BmN-SWU1 Cells" Insects 11, no. 10: 700. https://doi.org/10.3390/insects11100700

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