Next Article in Journal
First Insights into the Intrapuparial Development of Bactrocera dorsalis (Hendel): Application in Predicting Emergence Time for Tephritid Fly Control
Next Article in Special Issue
Insulin Receptor Substrate Gene Knockdown Accelerates Behavioural Maturation and Shortens Lifespan in Honeybee Workers
Previous Article in Journal
EAG Responses of Adult Lobesia botrana Males and Females Collected from Vitis vinifera and Daphne gnidium to Larval Host-Plant Volatiles and Sex Pheromone
Open AccessArticle

The Effect of Diet on the Composition and Stability of Proteins Secreted by Honey Bees in Honey

1
Institut für Biologie, Molekulare Ökologie, Martin-Luther-Universität Halle-Wittenberg, Hoher Weg 8, 06120 Halle (Saale), Germany
2
Institutul de Științele Vieții “Regele Mihai I al României”, Nutriție moleculară (Genomică și Proteomică), Universitatea de Științe Agricole și Medicină Veterinară, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania
3
Proteinzentrum Charles Tanford, Core Facility-Proteomic Mass Spectrometry, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Straße 3a, 06120 Halle (Saale), Germany
4
Institut für Biochemie und Biotechnologie, Pflanzenbiochemie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Straße 3a, 06120 Halle (Saale), Germany
5
Zentrum für Medizinische Grundlagenforschung (ZMG), Medizinische Fakultät der Martin-Luther-Universität Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally.
Insects 2019, 10(9), 282; https://doi.org/10.3390/insects10090282
Received: 17 July 2019 / Revised: 28 August 2019 / Accepted: 29 August 2019 / Published: 2 September 2019
(This article belongs to the Special Issue Mechanisms of Nutritional Resource Exploitation by Insects)
Honey proteins are essential bee nutrients and antimicrobials that protect honey from microbial spoilage. The majority of the honey proteome includes bee-secreted peptides and proteins, produced in specialised glands; however, bees need to forage actively for nitrogen sources and other basic elements of protein synthesis. Nectar and pollen of different origins can vary significantly in their nutritional composition and other compounds such as plant secondary metabolites. Worker bees producing and ripening honey from nectar might therefore need to adjust protein secretions depending on the quality and specific contents of the starting material. Here, we assessed the impact of different food sources (sugar solutions with different additives) on honey proteome composition and stability, using controlled cage experiments. Honey-like products generated from sugar solution with or without additional protein, or plant secondary metabolites, differed neither in protein quality nor in protein quantity among samples. Storage for 4 weeks prevented protein degradation in most cases, without differences between food sources. The honey-like product proteome included several major royal jelly proteins, alpha-glucosidase and glucose oxidase. As none of the feeding regimes resulted in different protein profiles, we can conclude that worker bees may secrete a constant amount of each bee-specific protein into honey to preserve this highly valuable hive product. View Full-Text
Keywords: Apis mellifera; major royal jelly proteins; invertase; diastase; glucose oxidase; honey production; honey ripening; mass spectrometry Apis mellifera; major royal jelly proteins; invertase; diastase; glucose oxidase; honey production; honey ripening; mass spectrometry
Show Figures

Graphical abstract

MDPI and ACS Style

Lewkowski, O.; Mureșan, C.I.; Dobritzsch, D.; Fuszard, M.; Erler, S. The Effect of Diet on the Composition and Stability of Proteins Secreted by Honey Bees in Honey. Insects 2019, 10, 282.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop