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Insects
Volume 10
Issue 9
10.3390/insects10090279
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Article

A Transcriptomics Approach Reveals Putative Interaction of Candidatus Liberibacter Solanacearum with the Endoplasmic Reticulum of Its Psyllid Vector

by
Saptarshi Ghosh
1,
Ola Jassar
1,
Svetlana Kontsedalov
1,
Galina Lebedev
1,
Chunxia Wang
2,
Donielle Turner
2,
Amit Levy
2,3 and
Murad Ghanim
1,*
1
Department of Entomology, the Volcani Center, Rishon LeZion 7505101, Israel
2
Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA
3
Department of Plant Pathology, University of Florida, Gainesville, FL 32601, USA
*
Author to whom correspondence should be addressed.
Insects 2019, 10(9), 279; https://doi.org/10.3390/insects10090279
Submission received: 16 July 2019 / Revised: 21 August 2019 / Accepted: 28 August 2019 / Published: 2 September 2019
(This article belongs to the Special Issue The Interactions between Plant Bacterial Pathogens and Their Insect Vectors)
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Abstract

Candidatus Liberibacter solanacerum (CLso), transmitted by Bactericera trigonica in a persistent and propagative mode causes carrot yellows disease, inflicting hefty economic losses. Understanding the process of transmission of CLso by psyllids is fundamental to devise sustainable management strategies. Persistent transmission involves critical steps of adhesion, cell invasion, and replication before passage through the midgut barrier. This study uses a transcriptomic approach for the identification of differentially expressed genes with CLso infection in the midguts, adults, and nymphs of B. trigonica and their putative involvement in CLso transmission. Several genes related to focal adhesion and cellular invasion were upregulated after CLso infection. Interestingly, genes involved with proper functionality of the endoplasmic reticulum (ER) were upregulated in CLso infected samples. Notably, genes from the endoplasmic reticulum associated degradation (ERAD) and the unfolded protein response (UPR) pathway were overexpressed after CLso infection. Marker genes of the ERAD and UPR pathways were also upregulated in Diaphorina citri when infected with Candidatus Liberibacter asiaticus (CLas). Upregulation of the ERAD and UPR pathways indicate induction of ER stress by CLso/CLas in their psyllid vector. The role of ER in bacteria–host interactions is well-documented; however, the ER role following pathogenesis of CLso/CLas is unknown and requires further functional validation.
Keywords: psyllid; bacterial pathogen; insect vector; Liberibacter; ER stress; ERAD; UPR psyllid; bacterial pathogen; insect vector; Liberibacter; ER stress; ERAD; UPR

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MDPI and ACS Style

Ghosh, S.; Jassar, O.; Kontsedalov, S.; Lebedev, G.; Wang, C.; Turner, D.; Levy, A.; Ghanim, M. A Transcriptomics Approach Reveals Putative Interaction of Candidatus Liberibacter Solanacearum with the Endoplasmic Reticulum of Its Psyllid Vector. Insects 2019, 10, 279. https://doi.org/10.3390/insects10090279

AMA Style

Ghosh S, Jassar O, Kontsedalov S, Lebedev G, Wang C, Turner D, Levy A, Ghanim M. A Transcriptomics Approach Reveals Putative Interaction of Candidatus Liberibacter Solanacearum with the Endoplasmic Reticulum of Its Psyllid Vector. Insects. 2019; 10(9):279. https://doi.org/10.3390/insects10090279

Chicago/Turabian Style

Ghosh, Saptarshi, Ola Jassar, Svetlana Kontsedalov, Galina Lebedev, Chunxia Wang, Donielle Turner, Amit Levy, and Murad Ghanim. 2019. "A Transcriptomics Approach Reveals Putative Interaction of Candidatus Liberibacter Solanacearum with the Endoplasmic Reticulum of Its Psyllid Vector" Insects 10, no. 9: 279. https://doi.org/10.3390/insects10090279

APA Style

Ghosh, S., Jassar, O., Kontsedalov, S., Lebedev, G., Wang, C., Turner, D., Levy, A., & Ghanim, M. (2019). A Transcriptomics Approach Reveals Putative Interaction of Candidatus Liberibacter Solanacearum with the Endoplasmic Reticulum of Its Psyllid Vector. Insects, 10(9), 279. https://doi.org/10.3390/insects10090279

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