Lethal Effects of High Temperatures on Brown Marmorated Stink Bug Adults before and after Overwintering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Lethal High Temperatures with Short Exposure Times
2.3. Nutrient Index and H. halys Mortality after High-Temperature Exposures
2.4. Data Analysis
3. Results
3.1. Lethal High Temperatures
3.2. Exposure Times at High Temperatures
3.3. Weight and Nutrient Index
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Adults’ State | Exposure Time (Minutes) | n | LT50 (°C) * | 95% CILT50 (°C) | LT99 (°C) * | 95% CILT99 (°C) | Intercept | SEIntercept | Slope | SESlope | Χ2 ** | df | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Lower | Upper | Lower | Upper | |||||||||||||
EXA | 2.5 | 310 | 52.04 | b | 51.54 | 52.50 | 56.07 | b | 55.22 | 57.39 | −30.01 | 3.70 | 0.58 | 0.07 | 32.94 | 29 |
ENA | 2.5 | 400 | 52.61 | a | 51.94 | 53.20 | 56.66 | a | 55.68 | 58.30 | −73.15 | 4.51 | 1.55 | 0.09 | 26.16 | 35 |
EXA | 15.0 | 310 | 44.60 | b | 43.69 | 45.09 | 47.17 | b | 46.33 | 49.81 | −40.47 | 11.49 | 0.91 | 0.25 | 8.77 | 29 |
ENA | 15.0 | 400 | 46.53 | a | 46.36 | 46.69 | 47.95 | a | 47.62 | 48.51 | −76.34 | 10.92 | 1.64 | 0.23 | 24.66 | 35 |
EXA | 30.0 | 310 | 43.44 | b | 42.86 | 43.96 | 46.29 | b | 45.44 | 48.01 | −35.44 | 6.81 | 0.82 | 0.16 | 10.97 | 29 |
ENA | 30.0 | 400 | 45.64 | a | 45.38 | 45.81 | 46.83 | a | 46.52 | 47.53 | −89.54 | 19.04 | 1.96 | 0.41 | 14.17 | 35 |
EXA | 60.0 | 310 | 41.34 | b | 40.23 | 42.05 | 45.39 | a | 44.26 | 47.94 | −23.74 | 5.31 | 0.57 | 0.13 | 11.01 | 29 |
ENA | 60.0 | 400 | 42.95 | a | 42.62 | 44.70 | 44.83 | b | 43.78 | 53.05 | −53.09 | 20.83 | 1.24 | 0.49 | 4.61 | 35 |
Adults’ State | Temperature (°C) | n | MT50 (Minutes) * | 95% CIMT50 (Minutes) | MT99 (Minutes) * | 95% CIMT99 (Minutes) | Intercept | SEIntercept | Slope | SESlope | Χ2 ** | df | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Lower | Upper | Lower | Upper | |||||||||||||
EXA | 47.5 | 400 | 8.67 | a | 7.80 | 9.54 | 15.65 | b | 14.06 | 18.13 | −2.89 | 0.38 | 0.33 | 0.04 | 33.28 | 43 |
ENA | 47.5 | 310 | 9.69 | a | 8.53 | 10.83 | 17.14 | a | 15.22 | 20.39 | −3.03 | 0.48 | 0.31 | 0.05 | 22.14 | 28 |
EXA | 50.0 | 400 | 3.72 | a | 3.31 | 4.09 | 6.10 | b | 5.50 | 7.12 | −3.64 | 0.61 | 0.98 | 0.15 | 9.05 | 34 |
ENA | 50.0 | 310 | 4.19 | a | 3.53 | 4.90 | 7.67 | a | 6.40 | 11.18 | −2.80 | 0.72 | 0.67 | 0.17 | 2.43 | 28 |
Source of Variation | 15 min | 30 min | 60 min | ||||||
---|---|---|---|---|---|---|---|---|---|
F Value | df | p-Value | F Value | df | p-Value | F Value | df | p-Value | |
Status (dead or alive) | 0.10 | 1; 210 | 0.7517 | 0.38 | 1; 210 | 0.5404 | 0.17 | 1; 210 | 0.6850 |
Temperature (Temp) | 3.47 | 1; 210 | 0.0639 | 2.47 | 1; 210 | 0.1173 | 1.20 | 1; 210 | 0.2739 |
Status × Temp | 0.50 | 1; 210 | 0.4804 | 0.66 | 1; 210 | 0.4173 | 0.00 | 1; 210 | 0.9954 |
Sex | 92.77 | 1; 210 | <0.0001 | 54.81 | 1; 210 | <0.0001 | 46.79 | 1; 210 | <0.0001 |
Status × Sex | 0.31 | 1; 210 | 0.5793 | 1.47 | 1; 210 | 0.2273 | 0.27 | 1; 210 | 0.6033 |
Sex × Temp | 0.46 | 1; 210 | 0.4968 | 0.27 | 1; 210 | 0.6066 | 0.83 | 1; 210 | 0.3621 |
Status × Sex × Temp | 0.14 | 1; 210 | 0.7090 | 0.73 | 1; 210 | 0.3950 | 0.18 | 1; 210 | 0.6732 |
Overwintering state (OS) | 22.12 | 1; 210 | <0.0001 | 13.88 | 1; 210 | 0.0003 | 16.35 | 1; 210 | <0.0001 |
Status × OS | 0.08 | 1; 210 | 0.7759 | 0.41 | 1; 210 | 0.5202 | 0.30 | 1; 210 | 0.5815 |
OS × Temp | 0.11 | 1; 210 | 0.7391 | 0.45 | 1; 210 | 0.5037 | 1.29 | 1; 210 | 0.2579 |
Status × OS × Temp | 0.51 | 1; 210 | 0.4763 | 0.21 | 1; 210 | 0.6442 | 1.86 | 1; 210 | 0.1744 |
Sex × OS | 0.76 | 1; 210 | 0.3844 | 1.84 | 1; 210 | 0.1765 | 1.37 | 1; 210 | 0.2439 |
Status × Sex × OS | 0.07 | 1; 210 | 0.7867 | 0.18 | 1; 210 | 0.6746 | 0.62 | 1; 210 | 0.4324 |
Sex × OS × Temp | 0.93 | 1; 210 | 0.3366 | 0.69 | 1; 210 | 0.4071 | 0.24 | 1; 210 | 0.6227 |
Status × Sex × OS × Temp | 0.03 | 1; 210 | 0.8633 | 0.27 | 1; 210 | 0.6029 | 0.01 | 1; 210 | 0.9118 |
Source of Variation | 15 min | 30 min | 60 min | ||||||
---|---|---|---|---|---|---|---|---|---|
F Value | df | p-Value | F Value | df | p-Value | F Value | df | p-Value | |
Status (dead or alive) | 0.14 | 1; 210 | 0.7120 | 0.00 | 1; 210 | 0.9877 | 0.69 | 1; 210 | 0.4066 |
Temperature (Temp) | 1.94 | 1; 210 | 0.1652 | 2.55 | 1; 210 | 0.1118 | 0.98 | 1; 210 | 0.3223 |
Status × Temp | 0.16 | 1; 210 | 0.6930 | 0.84 | 1; 210 | 0.3595 | 0.02 | 1; 210 | 0.9024 |
Sex | 1.34 | 1; 210 | 0.2481 | 2.87 | 1; 210 | 0.0915 | 3.88 | 1; 210 | 0.0501 |
Status × Sex | 0.03 | 1; 210 | 0.8519 | 1.65 | 1; 210 | 0.1999 | 1.93 | 1; 210 | 0.1657 |
Sex × Temp | 0.94 | 1; 210 | 0.3343 | 1.42 | 1; 210 | 0.2353 | 0.28 | 1; 210 | 0.6002 |
Status × Sex × Temp | 0.67 | 1; 210 | 0.4144 | 0.17 | 1; 210 | 0.6766 | 0.13 | 1; 210 | 0.7237 |
Overwintering state (OS) | 22.5 | 1; 210 | <0.0001 | 17.77 | 1; 210 | <0.0001 | 20.34 | 1; 210 | <0.0001 |
Status × OS | 0.02 | 1; 210 | 0.8758 | 0.90 | 1; 210 | 0.3431 | 1.03 | 1; 210 | 0.3110 |
OS × Temp | 0.29 | 1; 210 | 0.5913 | 0.81 | 1; 210 | 0.3701 | 0.49 | 1; 210 | 0.4832 |
Status × OS × Temp | 0.32 | 1; 210 | 0.5732 | 0.62 | 1; 210 | 0.4313 | 0.19 | 1; 210 | 0.6646 |
Sex × OS | 0.38 | 1; 210 | 0.5361 | 2.70 | 1; 210 | 0.1016 | 1.80 | 1; 210 | 0.1814 |
Status × Sex × OS | 0.01 | 1; 210 | 0.9237 | 0.42 | 1; 210 | 0.5154 | 0.28 | 1; 210 | 0.5991 |
Sex × OS × Temp | 3.88 | 1; 210 | 0.0501 | 1.80 | 1; 210 | 0.1817 | 0.53 | 1; 210 | 0.4692 |
Status × Sex × OS × Temp | 0.00 | 1; 210 | 0.9530 | 0.00 | 1; 210 | 0.979 | 0.46 | 1; 210 | 0.5000 |
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Scaccini, D.; Duso, C.; Pozzebon, A. Lethal Effects of High Temperatures on Brown Marmorated Stink Bug Adults before and after Overwintering. Insects 2019, 10, 355. https://doi.org/10.3390/insects10100355
Scaccini D, Duso C, Pozzebon A. Lethal Effects of High Temperatures on Brown Marmorated Stink Bug Adults before and after Overwintering. Insects. 2019; 10(10):355. https://doi.org/10.3390/insects10100355
Chicago/Turabian StyleScaccini, Davide, Carlo Duso, and Alberto Pozzebon. 2019. "Lethal Effects of High Temperatures on Brown Marmorated Stink Bug Adults before and after Overwintering" Insects 10, no. 10: 355. https://doi.org/10.3390/insects10100355
APA StyleScaccini, D., Duso, C., & Pozzebon, A. (2019). Lethal Effects of High Temperatures on Brown Marmorated Stink Bug Adults before and after Overwintering. Insects, 10(10), 355. https://doi.org/10.3390/insects10100355