Developing an Algorithm for Discriminating Oral Cancerous and Normal Tissues Using Raman Spectroscopy
Abstract
:1. Introduction
2. Methods and Materials
2.1. Patients and Samples
2.2. Development of Tissue Classification Models Using the Training Data Set
2.3. Validation of Tissue Classification Models Using a Validation Data Set
2.4. Pre-Processing of Spectra
2.5. Instrumentation
2.6. Univariate and Multivariate Analysis
3. Results and Discussion
3.1. Spectral Analysis
3.2. Characteristics of the Validation Set
3.3. Validation of the Classification Models
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Subsites | Tongue | Buccal Mucosa | Gingiva | Total |
---|---|---|---|---|
Tumor | 20 | 30 | 17 | 67 |
Normal | 18 | 29 | 17 | 64 |
Raman Bands (cm) | Compound/Assignments |
---|---|
749 | Symmetric Breathing of Tryptophan (Protein Assignment) |
848 | Tyrosine (protein assignment) |
1004 | Phenylalanine (ring breathing mode) |
1064 | Skeletal C-C stretch of lipids |
1123 | (C-N), proteins (protein assignment) |
1156 | C-C(and C-N) stretching of proteins/ carotenoid |
1168 | Lipids |
1220 | =CH bending (lipids) |
1302 | Amide III (protein) |
1450 | CH2 bending in proteins and lipids |
1517 | beta -carotene or porphyrin |
1650 | In normal tissue due to C=C of lipids and in tumor tissue due to |
Amide-I-proteins |
Dataset | Confusion Table | Performance Parameters | ||||
---|---|---|---|---|---|---|
PCA-LDA | Normal | Tumor | Total | Accuracy (%) | Sensitivity (%) | Specificity (%) |
Normal | 28 | 0 | 28 | 90.2 | 78.3 | 100 |
Tumor | 5 | 18 | 23 | |||
PLS-LDA | Normal | Tumor | Total | Accuracy (%) | Sensitivity (%) | Specificity (%) |
Normal | 28 | 0 | 28 | 100 | 100 | 100 |
Tumor | 0 | 23 | 23 |
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Sharma, M.; Jeng, M.-J.; Young, C.-K.; Huang, S.-F.; Chang, L.-B. Developing an Algorithm for Discriminating Oral Cancerous and Normal Tissues Using Raman Spectroscopy. J. Pers. Med. 2021, 11, 1165. https://doi.org/10.3390/jpm11111165
Sharma M, Jeng M-J, Young C-K, Huang S-F, Chang L-B. Developing an Algorithm for Discriminating Oral Cancerous and Normal Tissues Using Raman Spectroscopy. Journal of Personalized Medicine. 2021; 11(11):1165. https://doi.org/10.3390/jpm11111165
Chicago/Turabian StyleSharma, Mukta, Ming-Jer Jeng, Chi-Kuang Young, Shiang-Fu Huang, and Liann-Be Chang. 2021. "Developing an Algorithm for Discriminating Oral Cancerous and Normal Tissues Using Raman Spectroscopy" Journal of Personalized Medicine 11, no. 11: 1165. https://doi.org/10.3390/jpm11111165