Value of SERCA2a as a Biomarker for the Identification of Patients with Heart Failure Requiring Circulatory Support
Abstract
:1. Introduction and Objective
2. Material and Methods
2.1. Patients and Samples
2.2. Sample Processing
2.3. Specific Sandwich Enzyme-Linked Immunosorbent Assay
2.4. Statistical Analysis
3. Results
3.1. Clinical Characteristics of Patients
3.2. Plasma Levels of Molecules Involved in Intramyocardial Calcium Homeostasis and Nucleocytoplasmic Transport
3.3. SERCA2a Capacity to Predict Advanced HF with an Unstable Clinical Outcome
4. Discussion
5. Limitations
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
HT | Heart transplantation |
HF | Heart Failure |
MCS | Mechanical Circulatory Support |
NPC | Nuclear pore complex |
IMPs | Importins |
EXPs | Exportins |
SERCA2a | Sarcoplasmic reticulum Ca2+ ATPase |
IPO5 | Importin5 |
NUP153 | Nucleoporin153 kDa |
RanGAP1 | RanGTPase-Activating Protein 1 |
ECMO | Extracorporeal membrane oxygenator |
ELISAs | Enzyme-linked immunosorbent assays |
INTERMACS | Interagency Registry for Mechanically Assisted Circulatory Support |
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MCS (n = 13) | Non-MCS (n = 16) | p | |
---|---|---|---|
Age (years) | 52 ± 10 | 50 ± 14 | 0.60 |
AF (%) | 54 | 50 | 1.00 |
BMI (Kg/m2) | 26.4 ± 4 | 26.4 ± 3 | 0.90 |
CO (L/min) | 3.3 ± 0.4 | 3.6 ± 0.8 | 0.40 |
COPD (%) | 8 | 0 | 0.40 |
CRT (%) | 15 | 31 | 0.40 |
DM (%) | 15 | 12 | 1.00 |
eGFR (ml/min/1.73 m2) | 93 ± 33 | 70 ± 24 | 0.06 |
Gender (% Men) | 85 | 81 | 1.00 |
HBP (%) | 23 | 37 | 0.40 |
ICD (%) | 77 | 94 | 0.30 |
ICM (%) | 54 | 37 | 0.50 |
Inotropes (%) | 92% | 25% | 0.00 |
INTERMACS (%) | I–II: 15.4 | III: 18.8 | 0.00 |
II–III: 84.6 | IV–V: 81.3 | ||
LVEF (%) | 20 ± 7 | 27 ± 18 | 0.20 |
mPAP (mmHg) | 41 ± 12 | 34 ± 10 | 0.10 |
MV (%) | 23 | 0 | 0.08 |
PCWP (mmHg) | 28 ± 10 | 24 ± 8 | 0.40 |
PHT (%) | 90 | 81 | 0.90 |
Pr.CVS (%) | 8 | 12 | 0.90 |
Pr.Infection (%) | 31 | 12 | 0.40 |
Pr.VascD (%) | 15 | 12 | 1.00 |
PVR (UW) | 3.7 ± 2.4 | 2.8 ± 1.1 | 0.20 |
RI (%) | 31 | 25 | 0.90 |
Smoking (Yes/Ex) (%) | 8/54 | 12/37 | 0.70 |
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Ezzitouny, M.; Roselló-Lletí, E.; Portolés, M.; Sánchez-Lázaro, I.; Arnau-Vives, M.Á.; Tarazón, E.; Gil-Cayuela, C.; Lozano-Edo, S.; López-Vilella, R.; Almenar-Bonet, L.; et al. Value of SERCA2a as a Biomarker for the Identification of Patients with Heart Failure Requiring Circulatory Support. J. Pers. Med. 2021, 11, 1122. https://doi.org/10.3390/jpm11111122
Ezzitouny M, Roselló-Lletí E, Portolés M, Sánchez-Lázaro I, Arnau-Vives MÁ, Tarazón E, Gil-Cayuela C, Lozano-Edo S, López-Vilella R, Almenar-Bonet L, et al. Value of SERCA2a as a Biomarker for the Identification of Patients with Heart Failure Requiring Circulatory Support. Journal of Personalized Medicine. 2021; 11(11):1122. https://doi.org/10.3390/jpm11111122
Chicago/Turabian StyleEzzitouny, Meryem, Esther Roselló-Lletí, Manuel Portolés, Ignacio Sánchez-Lázaro, Miguel Ángel Arnau-Vives, Estefanía Tarazón, Carolina Gil-Cayuela, Silvia Lozano-Edo, Raquel López-Vilella, Luis Almenar-Bonet, and et al. 2021. "Value of SERCA2a as a Biomarker for the Identification of Patients with Heart Failure Requiring Circulatory Support" Journal of Personalized Medicine 11, no. 11: 1122. https://doi.org/10.3390/jpm11111122