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Open AccessArticle

Chronic Hindlimb Ischemia Assessment; Quantitative Evaluation Using Laser Doppler in a Rodent Model of Surgically Induced Peripheral Arterial Occlusion

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Division of Clinical Practical Skills, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timiș, Romania
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Department of Plastic, Aesthetic, Reconstructive and Hand Surgery, Evangelisches Krankenhaus Oldenburg, Medical Campus, University of Oldenburg, 26122 Oldenburg, Germany
3
Division of Microsurgery, Pius Branzeu Center for Laparoscopic Surgery and Microsurgery, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timiș, Romania
4
Neuromed Diagnostic Imaging Centre, Timisoara, 300218 Timiș, Romania
*
Author to whom correspondence should be addressed.
Diagnostics 2019, 9(4), 139; https://doi.org/10.3390/diagnostics9040139
Received: 17 September 2019 / Revised: 29 September 2019 / Accepted: 30 September 2019 / Published: 2 October 2019
(This article belongs to the Section Medical Imaging)
Therapeutic neoangiogenesis (TNA) holds promise as a treatment for peripheral arterial disease. Nevertheless, proper tools for in vivo pre-clinical investigation of different TNA approaches and their effects are still lacking. Here we describe a chronic ischemic hindlimb model in rats using laser Doppler quantitative evaluation of tissue perfusion. Male Wistar rats (n = 20), aged between 6–8 months, with an average weight of 287 ± 26.74 g, were used. Animals were divided into two experimental groups: group A (n = 17; hindlimb chronic ischemia model) and group B (n = 3; control). Hindlimb ischemia was induced by concomitant ligation of the right femoral and popliteal artery. Evaluation of tissue perfusion was quantified in perfusion units (PU) on a scale from 0 to 500 (500 PU = maximal detectable perfusion) by laser Doppler analysis at day 0, day 15 and day 30 after induction of ischemia. Induction of chronic ischemia in the rat hindlimb by concomitant ligation of the femoral and popliteal artery can be readily obtained but requires basic microsurgical skills. Laser Doppler analysis has shown unaltered ischemia levels throughout the study (129,17 PU ± 3.13 day 0 vs. 130,33 PU day 30 ± 3,27, p = not significant (n.s.)). We demonstrate a simple and reproducible model of chronic hindlimb ischemia in rats, with stable tissue perfusion levels that are accurately quantified using laser Doppler technology. Hence, this model can represent a valid tool for further studies involving therapeutic neoangiogenesis. View Full-Text
Keywords: experimental model; hindlimb; chronic ischemia; laser Doppler experimental model; hindlimb; chronic ischemia; laser Doppler
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MDPI and ACS Style

Hoinoiu, B.; Jiga, L.P.; Nistor, A.; Dornean, V.; Barac, S.; Miclaus, G.; Ionac, M.; Hoinoiu, T. Chronic Hindlimb Ischemia Assessment; Quantitative Evaluation Using Laser Doppler in a Rodent Model of Surgically Induced Peripheral Arterial Occlusion. Diagnostics 2019, 9, 139.

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