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Current and Emerging Methods of Antibiotic Susceptibility Testing

School of Mechanical Engineering, Korea University of Technology and Education, Cheonan, Chungnam 31253, Korea
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Diagnostics 2019, 9(2), 49;
Received: 18 March 2019 / Revised: 28 April 2019 / Accepted: 28 April 2019 / Published: 3 May 2019
(This article belongs to the Special Issue Diagnosis of Bacterial Pathogens)
PDF [2425 KB, uploaded 30 May 2019]


Antibiotic susceptibility testing (AST) specifies effective antibiotic dosage and formulates a profile of empirical therapy for the proper management of an individual patient’s health against deadly infections. Therefore, rapid diagnostic plays a pivotal role in the treatment of bacterial infection. In this article, the authors review the socio-economic burden and emergence of antibiotic resistance. An overview of the phenotypic, genotypic, and emerging techniques for AST has been provided and discussed, highlighting the advantages and limitations of each. The historical perspective on conventional methods that have paved the way for modern AST like disk diffusion, Epsilometer test (Etest), and microdilution, is presented. Several emerging methods, such as microfluidic-based optical and electrochemical AST have been critically evaluated. Finally, the challenges related with AST and its outlook in the future are presented. View Full-Text
Keywords: Antibiotic susceptibility tests; resistance; phenotypic; genotypic; bacteria Antibiotic susceptibility tests; resistance; phenotypic; genotypic; bacteria

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Khan, Z.A.; Siddiqui, M.F.; Park, S. Current and Emerging Methods of Antibiotic Susceptibility Testing. Diagnostics 2019, 9, 49.

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