Evaluation of Cardiac Function in Young Mdx Mice Using MRI with Feature Tracking and Self-Gated Magnetic Resonance Cine Imaging
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Preparation
2.2. Magnetic Resonance Imaging
2.3. MRI Data Analysis
2.4. Statistical Analysis
2.5. Histological Analysis and Immunostaining
3. Results
3.1. Animals’ Characteristics
3.2. Visual Evaluation of Cine Images of Short Axis, Two-Chamber, and Four-Chamber View
3.3. Cardiac Function in 8- and 12-Week-Old Mdx Mice vs. Age-Matched Controls
3.4. Strain Analysis by Feature-Tracking Method in 8- and 12-Week-Old Mdx Mice vs. Age-Matched Controls
3.5. H&E Staining and Immunostaining of Myocardium Tissue
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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8 Week | 12 Week | |||
---|---|---|---|---|
Control (n = 10) | Mdx (n = 10) | Control (n = 10) | Mdx (n = 10) | |
Body weight (g) | 25.6 ± 1.3 | 26.2 ± 1.2 | 28.3 ± 1.1 | 29.3 ± 1.6 |
Heart rate (bpm) | 321 ± 32 | 275± 23 ** | 334 ± 35 | 277 ± 22 ** |
Respiratory rate (brpm) | 73.4 ± 3.4 | 65.5 ± 6.6 | 76.2 ± 4.6 | 70 ± 9.4 |
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Ueda, J.; Saito, S. Evaluation of Cardiac Function in Young Mdx Mice Using MRI with Feature Tracking and Self-Gated Magnetic Resonance Cine Imaging. Diagnostics 2023, 13, 1472. https://doi.org/10.3390/diagnostics13081472
Ueda J, Saito S. Evaluation of Cardiac Function in Young Mdx Mice Using MRI with Feature Tracking and Self-Gated Magnetic Resonance Cine Imaging. Diagnostics. 2023; 13(8):1472. https://doi.org/10.3390/diagnostics13081472
Chicago/Turabian StyleUeda, Junpei, and Shigeyoshi Saito. 2023. "Evaluation of Cardiac Function in Young Mdx Mice Using MRI with Feature Tracking and Self-Gated Magnetic Resonance Cine Imaging" Diagnostics 13, no. 8: 1472. https://doi.org/10.3390/diagnostics13081472