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Article

Pilot Testing of the “Turbidimeter”, a Simple, Universal Reader Intended to Complement and Enhance Bacterial Growth Detection in Manual Blood Culture Systems in Low-Resource Settings

1
Clinical Sciences Department, Institute of Tropical Medicine, 2000 Antwerp, Belgium
2
Center for Nano- and Biophotonics (NB-Photonics), Ghent University, 9000 Ghent, Belgium
3
Photonics Research Group, Department of Information Technology, Ghent University-imec, 9000 Ghent, Belgium
4
Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Chao-Min Cheng
Diagnostics 2022, 12(3), 615; https://doi.org/10.3390/diagnostics12030615
Received: 31 January 2022 / Revised: 18 February 2022 / Accepted: 25 February 2022 / Published: 1 March 2022
(This article belongs to the Special Issue Point-of-Care Diagnostics for Low-Resource Settings)
Bloodstream infections and antimicrobial resistance are an increasing problem in low-income countries. There is a clear need for adapted diagnostic tools. To address this need, we developed a simple, universal reader prototype that detects bacterial growth in blood culture bottles. Our “turbidimeter” evaluates bacterial growth, based on the turbidity of the broth and the color change of the colorimetric CO2 indicator in commercially available blood culture bottles. A total of 60 measurements were performed using 10 relevant microbial species, spiked in horse blood, to compare the turbidimeter’s performance with that of an automatic reference system. The turbidimeter was able to detect growth in all but one of the spiked blood culture bottles. In the majority (7/10) of the species tested, time-to-detection of the turbidimeter was shown to be non-inferior to the reference automated time-to-detection. This was, however, only the case when both the turbidity and color change in the colorimetric CO2-indicator were used to evaluate growth. We could not demonstrate the non-inferiority of the turbidity measurement alone. Overall, the turbidimeter performed well, but we also identified some improvements that will be implemented in the next version of the prototype. View Full-Text
Keywords: blood culture; bloodstream infection; bacterial growth; turbidimetry; low-resource settings blood culture; bloodstream infection; bacterial growth; turbidimetry; low-resource settings
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MDPI and ACS Style

Barbé, B.; Corsmit, E.; Jans, J.; Kaur, K.; Baets, R.; Jacobs, J.; Hardy, L. Pilot Testing of the “Turbidimeter”, a Simple, Universal Reader Intended to Complement and Enhance Bacterial Growth Detection in Manual Blood Culture Systems in Low-Resource Settings. Diagnostics 2022, 12, 615. https://doi.org/10.3390/diagnostics12030615

AMA Style

Barbé B, Corsmit E, Jans J, Kaur K, Baets R, Jacobs J, Hardy L. Pilot Testing of the “Turbidimeter”, a Simple, Universal Reader Intended to Complement and Enhance Bacterial Growth Detection in Manual Blood Culture Systems in Low-Resource Settings. Diagnostics. 2022; 12(3):615. https://doi.org/10.3390/diagnostics12030615

Chicago/Turabian Style

Barbé, Barbara, Ellen Corsmit, Jasper Jans, Kamalpreet Kaur, Roel Baets, Jan Jacobs, and Liselotte Hardy. 2022. "Pilot Testing of the “Turbidimeter”, a Simple, Universal Reader Intended to Complement and Enhance Bacterial Growth Detection in Manual Blood Culture Systems in Low-Resource Settings" Diagnostics 12, no. 3: 615. https://doi.org/10.3390/diagnostics12030615

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