The Eutopic Endometrium Proteome in Endometriosis Reveals Candidate Markers and Molecular Mechanisms of Physiopathology
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Sample Preparation
2.3. Protein Extraction, Digestion, and Liquid Chromatography—Tandem Mass Spectrometry (LC-MS/MS) Analyses
2.4. MS/MS Data Analysis
2.5. Statistical Analysis and Data Mining
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Inclusion Criterion | Exclusion Criterion | |
---|---|---|
♀ ⟪endometriosis⟫ N = 4 | Endometriosis (with symptoms) Histologic confirmation of endometriosis No hormonal treatment Laparoscopy between day 19 and 24 of menstrual cycle Presence of superficial and active lesions with angiogenesis around Presence of characteristic lesions in Pouch of Douglas | Adenomyosis No symptom Hormonal treatment Other day of menstrual cylce Hydrosalpinx |
♀ ⟪control⟫ N = 5 | Underwent surgery for infertility No pain symptom No hormonal treatment Laparoscopy between day 19 and 24 of menstrual cycle No visible lesion with ultrasound No cyst | Hydrosalpinx Endometriosis or other disease with effect on endometrium Hormonal treatment Other day of menstrual cycle |
UniProt iD. | Protein Names | Gene Names | Méar et al. Endometrium (Secretory) | Manousopoulou et al. Endometrium (Proliferative) | Manousopoulou et al. Serum |
---|---|---|---|---|---|
Q9Y446 | Plakophilin-3 | PKP3 | up | up | - |
A0AV96 | RNA-binding protein 47 | RBM47 | up | up | - |
Q9P2M7 | Cingulin | CGN | up | up | - |
O95210 | Starch-binding domain-containing protein 1 | STBD1 | up | up | - |
Q9UIL1 | Short coiled-coil protein | SCOC | up | up | - |
O00292 | Left-right determination factor 2 | LEFTY2 | up | up | - |
P13688 | Carcinoembryonic antigen-related cell adhesion molecule 1 | CEACAM1 | up | up | - |
P22748 | Carbonic anhydrase 4 | CA4 | up | up | - |
Q06710 | Paired box protein Pax-8 | PAX8 | up | up | - |
Q12974 | Protein tyrosine phosphatase type IVA 2 | PTP4A2 | up | up | - |
Q76KP1 | N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1 | B4GALNT4 | up | up | - |
P11047 | Laminin subunit gamma-1 | LAMC1 | up | - | up |
Q14203 | Dynactin subunit 1 | DCTN1 | up | - | up |
Q6IBS0 | Twinfilin-2 | TWF2 | up | - | up |
O00429 | Dynamin-1-like protein | DNM1L | up | - | up |
Q15942 | Zyxin | ZYX | up | - | up |
Q14247 | Src substrate cortactin | CTTN | up | - | up |
P12081 | Histidine—tRNA ligase, cytoplasmic | HARS | up | - | up |
O14974 | Protein phosphatase 1 regulatory subunit 12A | PPP1R12A | up | - | up |
Q27J81 | Inverted formin-2 | INF2 | up | - | up |
P14317 | Hematopoietic lineage cell-specific protein | HCLS1 | up | - | up |
O43294 | Transforming growth factor beta-1-induced transcript 1 protein | TGFB1I1 | up | - | up |
Q99426 | Tubulin-folding cofactor B | TBCB | up | - | up |
O95747 | Serine/threonine-protein kinase OSR1 | OXSR1 | up | - | up |
Q05209 | Tyrosine-protein phosphatase non-receptor type 12 | PTPN12 | up | - | up |
Q8N392 | Rho GTPase-activating protein 18 | ARHGAP18 | up | - | up |
P98196 | Probable phospholipid-transporting ATPase IH | ATP11A | up | - | up |
P08311 | Cathepsin G | CTSG | down | - | down |
P06702 | Protein S100-A9 | S100A9 | down | down | - |
P05109 | Protein S100-A8 | S100A8 | down | down | - |
P02775 | Platelet basic protein | PPBP | down | down | - |
P80511 | Protein S100-A12 | S100A12 | down | down | - |
P02743 | Serum amyloid P-component | APCS | down | down | - |
P02776 | Platelet factor 4 | PF4 | down | down | - |
P02788 | Lactotransferrin | LTF | down | down | down |
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Méar, L.; Com, E.; Fathallah, K.; Guillot, L.; Lavigne, R.; Guével, B.; Fauconnier, A.; Vialard, F.; Pineau, C. The Eutopic Endometrium Proteome in Endometriosis Reveals Candidate Markers and Molecular Mechanisms of Physiopathology. Diagnostics 2022, 12, 419. https://doi.org/10.3390/diagnostics12020419
Méar L, Com E, Fathallah K, Guillot L, Lavigne R, Guével B, Fauconnier A, Vialard F, Pineau C. The Eutopic Endometrium Proteome in Endometriosis Reveals Candidate Markers and Molecular Mechanisms of Physiopathology. Diagnostics. 2022; 12(2):419. https://doi.org/10.3390/diagnostics12020419
Chicago/Turabian StyleMéar, Loren, Emmanuelle Com, Khadija Fathallah, Laetitia Guillot, Régis Lavigne, Blandine Guével, Arnaud Fauconnier, François Vialard, and Charles Pineau. 2022. "The Eutopic Endometrium Proteome in Endometriosis Reveals Candidate Markers and Molecular Mechanisms of Physiopathology" Diagnostics 12, no. 2: 419. https://doi.org/10.3390/diagnostics12020419