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Article

A Novel System for Semiautomatic Sample Processing in Chronic Myeloid Leukaemia: Increasing Throughput without Impacting on Molecular Monitoring at Time of SARS-CoV-2 Pandemic

1
Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy
2
Center of Experimental Oncology and Hematology, A.O.U. Policlinico “G.Rodolico—San Marco”, 95123 Catania, Italy
3
Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, 90121 Palermo, Italy
4
Division of Hematology and Bone Marrow Transplant, A.O.U. Policlinico “G.Rodolico—San Marco”, 95123 Catania, Italy
5
Hematology Department of Surgery, Medical and Surgical Specialities, University of Catania, 95123 Catania, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Gaetano Magro, Paolo G. Vigneri and Rosalba Parenti
Diagnostics 2021, 11(8), 1502; https://doi.org/10.3390/diagnostics11081502
Received: 30 June 2021 / Revised: 17 August 2021 / Accepted: 19 August 2021 / Published: 20 August 2021
Molecular testing of the BCR-ABL1 transcript via real-time quantitative-polymerase-chain-reaction is the most sensitive approach for monitoring the response to tyrosine-kinase-inhibitors therapy in chronic myeloid leukaemia (CML) patients. Each stage of the molecular procedure has been standardized and optimized, including the total white blood cells (WBCs) and RNA isolation methods. Here, we compare the performance of our current manual protocol to a newly semiautomatic method based on the Biomek i-5 Automated Workstations integrated with the CytoFLEX Flow Cytometer, followed by the automatic QIAsymphony system to facilitate high-throughput processing samples and reduce the hands-on time and the risk associated with SARS-CoV-2. The recovery efficiency was investigated in blood samples from 100 adults with CML. We observe a 100% of concordance between the two methods, with similar total WBCs isolated (median 1.137 × 106 for manual method vs. 1.076 × 106 for semiautomatic system) and a comparable quality and quantity of RNA extracted (median 103 ng/μL with manual isolation kit vs. 99.95 ng/μL with the QIAsymphony system). Moreover, by stratifying patients according to their BCR-ABL1 transcript levels, we obtained similar BCR-ABL1/ABL1IS values and ABL1 copies, and matched samples were assigned to the same group of molecular response. We conclude that this newly semiautomatic workflow has a performance comparable to our more laborious standard manual, which can be replaced, particularly when specimens from patients with suspected or confirmed SARS-CoV-2 infection need to be processed. View Full-Text
Keywords: chronic myeloid leukaemia; molecular response; Q-PCR; SARS-CoV-2 infection; BCR-ABL1/ABL1IS chronic myeloid leukaemia; molecular response; Q-PCR; SARS-CoV-2 infection; BCR-ABL1/ABL1IS
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MDPI and ACS Style

Stella, S.; Vitale, S.R.; Massimino, M.; Puma, A.; Tomarchio, C.; Pennisi, M.S.; Tirrò, E.; Romano, C.; Martorana, F.; Stagno, F.; Di Raimondo, F.; Manzella, L. A Novel System for Semiautomatic Sample Processing in Chronic Myeloid Leukaemia: Increasing Throughput without Impacting on Molecular Monitoring at Time of SARS-CoV-2 Pandemic. Diagnostics 2021, 11, 1502. https://doi.org/10.3390/diagnostics11081502

AMA Style

Stella S, Vitale SR, Massimino M, Puma A, Tomarchio C, Pennisi MS, Tirrò E, Romano C, Martorana F, Stagno F, Di Raimondo F, Manzella L. A Novel System for Semiautomatic Sample Processing in Chronic Myeloid Leukaemia: Increasing Throughput without Impacting on Molecular Monitoring at Time of SARS-CoV-2 Pandemic. Diagnostics. 2021; 11(8):1502. https://doi.org/10.3390/diagnostics11081502

Chicago/Turabian Style

Stella, Stefania, Silvia R. Vitale, Michele Massimino, Adriana Puma, Cristina Tomarchio, Maria S. Pennisi, Elena Tirrò, Chiara Romano, Federica Martorana, Fabio Stagno, Francesco Di Raimondo, and Livia Manzella. 2021. "A Novel System for Semiautomatic Sample Processing in Chronic Myeloid Leukaemia: Increasing Throughput without Impacting on Molecular Monitoring at Time of SARS-CoV-2 Pandemic" Diagnostics 11, no. 8: 1502. https://doi.org/10.3390/diagnostics11081502

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