miRNomic Signature in Very Low Birth-Weight Neonates Discriminates Late-Onset Gram-Positive Sepsis from Controls
Abstract
:1. Introduction
2. Materials and Methods
2.1. The Study Design and Patients’ Characteristics
2.2. RNA Extraction
2.3. Small Non-Coding RNAs and mRNA Expression Profiling
2.4. Data Analysis of Microarrays
2.5. Data Analysis of Clinical and Demographic Analysis
3. Results
3.1. Patients’ Characteristics
3.2. Principal Component Analysis (PCA) of Neonatal miRNome
3.3. miRNome Differential Analysis
3.4. Combined Analysis: miRNAs Susceptible to Regulate mRNAs in Gram-Positive Sepsis versus Controls Neonates
3.5. Biological Analysis from a Combined Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Risk Factors of Sepsis |
|
Clinical Signs of Sepsis |
|
Gram-Positive Sepsis (n = 11) | Non-Septic Controls (n = 16) | p-Value | |
---|---|---|---|
Gestational age (weeks) | 26 (26–29) | 29 (27–30) | 0.22 a |
Gender: male | 5 (45.5) | 10 (62.5) | 0.38 b |
Birth weight (g) | 940 (868–1175) | 1270 (879–1445) | 0.29 a |
Ethnicity: Caucasian | 7 (63.6) | 15 (93.7) | 0.12 b |
Delivery: | 0.053 b | ||
Vaginal | 6 (54.5) | 3 (18.7) | |
C-Section | 5 (45.5) | 13 (81.2) | |
Apgar 1 min | 8 (5–9) | 6 (5–7) | 0.18 a |
Apgar 5 min | 9 (9–10) | 9 (8–9) | 0.63 a |
Nutrition: Breastmilk | 11 (100) | 15 (93.7) | 1 b |
Central line | 8 (72.7) | 8 (50) | 0.24 b |
Days after birth at sample collection | 11 (7–19) | 14 (3–26) | 0.72 a |
Weight at sample collection (g) | 1020 (875–1220) | 1210 (888–1473) | 0.23 a |
Transcript ID (Array Design) | p-Value (SEPSIS vs. CONTROL) | Fold Change (SEPSIS vs. CONTROL) |
---|---|---|
hsa-miR-31-5p | 0.0017 | −3.05 |
hsa-miR-1271-5p | 0.0089 | −2.77 |
hsa-miR-326 | 0.0013 | −2.39 |
hsa-miR-146b-5p | 0.0050 | −2.38 |
hsa-miR-140-5p | 0.0091 | −2.26 |
hsa-miR-409-5p | 0.0084 | −2.24 |
hsa-miR-668-3p | 0.0038 | −2.21 |
hsa-miR-27b-3p | 0.0028 | −2.12 |
hsa-miR-28-5p | 0.0019 | −2.06 |
hsa-miR-152-3p | 0.0080 | −1.95 |
hsa-miR-431-5p | 0.0094 | −1.95 |
hsa-miR-106b-5p | 0.0063 | −1.73 |
hsa-miR-151a-3p | 0.0002 | −1.72 |
hsa-miR-15a-5p | 0.0046 | −1.71 |
hsa-miR-339-5p | 0.0068 | −1.70 |
hsa-miR-30b-5p | 0.0079 | −1.68 |
hsa-miR-146a-5p | 0.0024 | −1.68 |
hsa-miR-20a-5p | 0.0008 | −1.64 |
hsa-miR-20b-5p | 0.0005 | −1.60 |
hsa-miR-532-5p | 0.0059 | −1.51 |
hsa-miR-30c-5p | 0.0044 | −1.47 |
hsa-miR-106a-5p | 0.0006 | −1.43 |
hsa-miR-17-5p | 0.0007 | −1.39 |
hsa-miR-23b-3p | 0.0017 | −1.37 |
hsa-miR-93-5p | 0.0088 | −1.22 |
hsa-miR-425-5p | 0.0083 | −1.21 |
hsa-miR-1298-5p | 0.0054 | −1.19 |
hsa-miR-107 | 0.0009 | −1.14 |
hsa-miR-103a-3p | 0.0004 | −1.14 |
hsa-miR-372-3p | 0.0054 | 1.11 |
hsa-miR-760 | 0.0063 | 1.34 |
hsa-miR-6088 | 0.0011 | 1.68 |
hsa-let-7c-5p | 0.0089 | 2.09 |
Biological Processes | p-Value | Number of Genes |
---|---|---|
Treg-Cell Differentiation | 4.92 × 10−9 | 27 |
Th2-Cell Differentiation | 1.84 × 10−8 | 26 |
Nociception Expression Targets Signaling | 5.62 × 10−8 | 43 |
mTOR Signaling | 2.80 × 10−7 | 42 |
Serotonin Receptors Signaling | 3.06 × 10−7 | 23 |
Catecholamines Secretion from Adrenal Gland | 5.00 × 10−7 | 26 |
Peripheral T-Cell Tolerance | 6.81 × 10−7 | 23 |
Vascular Motility | 7.59 × 10−7 | 28 |
Th1-Cell Differentiation | 1.06 × 10−6 | 24 |
Thrombopoietin Receptors Signaling in Platelet Maturation | 1.71 × 10−6 | 16 |
Subnetworks | p-Value | Number of Genes |
---|---|---|
Protein targets of proteasome endopeptidase complex | 1.4 × 10−213 | 266 |
Protein targets of TNF | 5.6 × 10−213 | 315 |
Protein targets of TGFB1 | 4.4 × 10−212 | 308 |
Protein targets of SP1 | 5.7 × 10−193 | 263 |
Protein targets of NF-kB family | 4.8 × 10−171 | 247 |
Protein targets of mitogen-activated protein kinase | 2.2 × 10−154 | 205 |
Protein targets of TP53 | 3.3 × 10−145 | 200 |
Protein targets of ubiquitin | 1.2 × 10−139 | 172 |
Protein targets of INS | 1.0 × 10−134 | 193 |
Protein targets of MAPK1 | 1.5 × 10−134 | 186 |
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Serna, E.; Parra-Llorca, A.; Panadero, J.; Vento, M.; Cernada, M. miRNomic Signature in Very Low Birth-Weight Neonates Discriminates Late-Onset Gram-Positive Sepsis from Controls. Diagnostics 2021, 11, 1389. https://doi.org/10.3390/diagnostics11081389
Serna E, Parra-Llorca A, Panadero J, Vento M, Cernada M. miRNomic Signature in Very Low Birth-Weight Neonates Discriminates Late-Onset Gram-Positive Sepsis from Controls. Diagnostics. 2021; 11(8):1389. https://doi.org/10.3390/diagnostics11081389
Chicago/Turabian StyleSerna, Eva, Anna Parra-Llorca, Joaquín Panadero, Máximo Vento, and María Cernada. 2021. "miRNomic Signature in Very Low Birth-Weight Neonates Discriminates Late-Onset Gram-Positive Sepsis from Controls" Diagnostics 11, no. 8: 1389. https://doi.org/10.3390/diagnostics11081389